Total Protein Reagent can be used for the quantitative, colorimetric determination of total protein concentration in solution at 540 nm. The total protein reagent is supplied as a ready-to-use liquid.
Total Protein Reagent has been used in the biuret assay.[1][2]
Azioni biochim/fisiol
A biuret-based total protein reagent. Copper ions in the alkaline reagent react with peptide bonds in proteins resulting in a purple color with an absorbance maximum of 540 nm.
We developed a candidate Reference Method for measuring total serum protein by use of the biuret reaction. The method involves a previously described biuret reagent (Clin. Chem. 21: 1159, 1975) and Standard Reference Material (SRM) 927 bovine albumin (National Bureau
Determination of serum proteins by means of the biuret reagent.
Gornall, A. G., et al.,
The Journal of Biological Chemistry, 177, 759-759 (1949)
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Systematic comparisons of various spectrophotometric and colorimetric methods to measure concentrations of protein, peptide and amino acid: detectable limits, linear dynamic ranges, interferences, practicality and unit costs.
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