SRP6169

IL-1β human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Sinonimo/i: Catabolin, Endogenous pyrogen (EP), Leukocyte endogenous mediator (LEM), Lymphocyte-activating factor (LAF), Mononuclear cell factor (MCF)
• Numero CAS: 97599-21-8
• Codice UNSPSC: 12352202
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in HEK 293 cells
• Saggio: ≥95% (SDS-PAGE)
• Stato: lyophilized
• PM: (18 and 25 kDa, monomer, glycosylated)
• Confezionamento: pkg of 10 μg
• tecniche: cell culture | mammalian: suitable
• N° accesso NCBI: P01584
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... IL1B(3553)

Descrizione

Descrizione generale

Interleukin-1β (IL1B) is produced by activated macrophages.[1] It is synthesized as pro-IL-1β which is changed to active form IL-1β with the help of pro-inflammatory protease caspase-1.[2] IL-1β belongs to the IL-1 gene family.[3] It is a proinflammatory cytokine.[4] The gene is mapped to human chromosome 2q14.[5] In comparison with the Escherichia coli expressed protein, IL-1β is 50% more potent in promoting human CD4+ T cell differentiation into Th17 (T helper) cells. This cytokine is produced in a serum free, chemically defined media.

Applicazioni

• IL-1β (IL1B) has been used to check the expression of NGAL (neutrophil gelatinase-associated lipocalin) in epithelial cells after stimulation with proinflammatory cytokines.[6]
• It has been used to check anti-inflammatory activities of N-Acetylglucosamine in human chondrocytes.[7]
• It has been used to create inflammatory endothelium in human coronary artery endothelial cells.[8]

Azioni biochim/fisiol

IL-1β (IL1B) stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation and fibroblast growth factor activity.[1] IL-1β proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.[9][10] IL-1β is present in chronic inflamed tissues and also in tissues going for fibrogenesis.[3] It brings neutrophil and macrophage to the site of infection.[11] Mutations in IL-1β gene are linked with renal manifestations and renal sequelae in Henoch-Schönlein purpura.[12] It is also associated with destruction of β-cell in type 1 diabetes.[13]

Stato fisico

Lyophilized from a PBS solution.

Nota sulla preparazione

Centrifuge the vial prior to opening.

Ricostituzione

Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable