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SRP3238

Sigma-Aldrich

EGF from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Sinonimo/i:

Epidermal Growth Factor, URG, Urogastrone

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100 μG
CHF 313.00

CHF 313.00


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Cambia visualizzazione
100 μG
CHF 313.00

About This Item

Codice UNSPSC:
12352202
NACRES:
NA.32

CHF 313.00


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Origine biologica

rat

Ricombinante

expressed in E. coli

Saggio

≥98% (HPLC)
≥98% (SDS-PAGE)

Stato

lyophilized

Potenza

≤0.1 ng/mL

PM

6.2 kDa

Confezionamento

pkg of 100 μg

tecniche

cell culture | mammalian: suitable

Impurezze

<0.1 EU/μg endotoxin, tested

Colore

white to off-white

N° accesso UniProt

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Informazioni sul gene

rat ... EGF(25313)

Descrizione generale

Epidermal growth factor (EGF) is a potent growth factor and a cytoprotective peptide.[1] Recombinant rat EGF is a 6.2kDa globular protein containing 54 amino acid residues, including 3 intramolecular disulfide-bonds.

Applicazioni

Epidermal growth factor (EGF) is used in hepatocyte culture to determine the optimal condition for survival and function of rat hepatocytes.

Azioni biochim/fisiol

Epidermal growth factor (EGF) stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, inflammation and is involved in wound healing.[2][3][1] EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor.[4] This receptor also binds with TGF-α (transforming growth factor-α) and VGF (vaccinia virus growth factor).

Sequenza

MNSNTGCPPS YDGYCLNGGV CMYVESVDRY VCNCVIGYIG ERCQHRDLRW WKLR

Stato fisico

Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5.

Ricostituzione

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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The role of epidermal growth factor in prevention of oxidative injury and apoptosis induced by intestinal ischemia/reperfusion in rats.
Arda-Pirinci P
Acta Histochemica, 116(1), 167-175 (2014)
The epidermal growth factor receptor family: biology driving targeted therapeutics.
Wieduwilt MJ and Moasser MM
Cellular and Molecular Life Sciences, 65(10), 1566-1584 (2008)
Epidermal growth factor improves survival and prevents intestinal injury in a murine model of pseudomonas aeruginosa pneumonia.
Dominguez JA
Shock, 36(4), 381-389 (2011)
Sohini Sen et al.
International journal of biological macromolecules, 143, 1009-1032 (2019-10-28)
Few studies are reported on immunomodulatory potential of non-mulberry (Antheraea mylitta) silk fibroin (SF) combined with polyurethane (PU) in diabetic wound healing. In this study, PU/SF (Antheraea mylitta) scaffolds were fabricated by blending and immobilization techniques. Effective SF dosage was
Lluïsa Miró et al.
American journal of physiology. Cell physiology, 304(9), C918-C926 (2013-03-08)
In vivo studies show that raised aldosterone (Aldo) during low-Na adaptation regulates the growth of pericryptal myofibroblasts and reduces the permeability of the colonic epithelium. The aim of this study was to reproduce in vitro the in vivo condition of

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