SRP0212

PTP1B (1-321)/PTPN1 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Sinonimo/i: PTPN1, Protein tyrosine phosphatase, non-receptor type 1
• Codice UNSPSC: 12352200
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in E. coli
• Saggio: ≥90% (SDS-PAGE)
• Forma fisica: aqueous solution
• PM: 63.3 kDa
• Confezionamento: pkg of 20 μg
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• Concentrazione: 0.84 mg/mL
• N° accesso NCBI: M31724
• N° accesso UniProt: P18031
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... PTPN1(5770)

Descrizione

Descrizione generale

Protein tyrosine phosphatase, catalytic domain, (amino acids 1-321), (Gene bank accession no. M31724) with N-terminal GST tag, MW = 63.3 kDa, expressed in an E. coli expression system.

Protein tyrosine phosphatase, non-receptor type 1 (PTPN1), which is also known as protein-tyrosine phosphatase 1B (PTP1B), is a tyrosine phosphatase. It is localized at the cytoplasmic side of the endoplasmic reticulum. The gene encoding PTPN1 is localized on human chromosome 20q13.13.

Applicazioni

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

Azioni biochim/fisiol

Protein tyrosine phosphatase, non-receptor type 1 (PTPN1) or protein-tyrosine phosphatase 1B (PTP1B) dephosphorylates the epidermal growth factor receptor. It acts as a modulator of insulin-like growth factor receptor (IGFR) and MET-mediated signaling responses. It may be useful as a drug target in type 2 diabetes and obesity. The protein has been shown to enhance tumor progression in various cancers such as breast, colon and prostate. It acts a tumor suppressor in other cancers (lymphomas and esophageal cancers).

Definizione di unità

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Stato fisico

Formulated in 25 Mm Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Nota sulla preparazione

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable