SRP0211

PTP-D2/PTPN14 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Sinonimo/i: Cytoskeletal-associated protein tyrosine phosphatase, PTP36, PTPN14, Pez, Protein tyrosine phosphatase, non-receptor type 14
• Codice UNSPSC: 12352200
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in E. coli
• Saggio: ≥90% (SDS-PAGE)
• Stato: aqueous solution
• PM: 61 kDa
• Confezionamento: pkg of 20 μg
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• Concentrazione: >0.02 mg/mL
• N° accesso NCBI: NM_005401
• N° accesso UniProt: Q15678
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... PTPN14(5784)

Descrizione

Descrizione generale

Catalytic domain, (amino acids 817-1188), (Gene bank accession number NM_005401) with N-terminal GST tag, MW = 61 kDa, expressed in an E. coli expression system.

Applicazioni

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

Definizione di unità

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Stato fisico

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Nota sulla preparazione

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.