SML0382

KU 0063794

≥98% (HPLC)

• Sinonimo/i: rel-5-[2-[(2R,6S)-2,6-Dimethyl-4-morpholinyl]-4-(4-morp holinyl)pyrido[2,3-d]pyrimidin-7-yl]-2-methoxybenzenemethanol
• Formula empirica (notazione di Hill): C₂₅H₃₁N₅O₄
• Numero CAS: 938440-64-3
• Peso molecolare: 465.54
• Numero MDL: MFCD11977741
• Codice UNSPSC: 12352200
• ID PubChem: 329825382
• NACRES: NA.77

Proprietà

• Saggio: ≥98% (HPLC)
• Stato: powder
• Colore: white to beige
• Solubilità: DMSO: >2 mg/mL (warmed)
• Temperatura di conservazione: 2-8°C
• Stringa SMILE: COc1ccc(cc1CO)-c2ccc3c(nc(nc3n2)N4C[C@H](C)O[C@H](C)C4)N5CCOCC5
• InChI: 1S/C25H31N5O4/c1-16-13-30(14-17(2)34-16)25-27-23-20(24(28-25)29-8-10-33-11-9-29)5-6-21(26-23)18-4-7-22(32-3)19(12-18)15-31/h4-7,12,16-17,31H,8-11,13-15H2,1-3H3/t16-,17+; RFSMUFRPPYDYRD-CALCHBBNSA-N

Descrizione

Applicazioni

KU 0063794 has been used:

• as a mammalian target of rapamycin (mTOR) inhibitor to study the effects of follicular stimulating hormone (FSH) in mTOR phosphorylation and vascular cell adhesion molecule-1 (VCAM-1) expression in human umbilical vascular endothelial cells (HUVECs)[1]
• as a mTOR inhibitor to treat effector memory (EM) CD8⁺ T cells for metabolic flux analysis[2]
• as an autophagy inducer to demonstrate the utility of p62 and LC3B-II quantification in HEK293T cells and primary cultures of rat neurons and astrocytes using time-resolved fluorescence resonance energy transfer (TR-FRET)[3]

Azioni biochim/fisiol

KU 0063794 induces autophagy.[3] It is cell-permeant and suppresses activation and hydrophobic motif phosphorylation of protein kinase B (Akt), p70 ribosomal S6 kinase (S6K) and serum and glucocorticoid protein kinase (SGK).[4]

KU 0063794 is a potent and selective inhibitor of mammalian target of rapamycin (mTOR) (IC₅₀ ~10 nM for both mTORC1 and mTORC2) that inhibits both mTORC1 and mTORC2 in vitro and in vivo (IC₅₀ ~10 nM for both mTORC1 and mTORC2). KU 0063794 does not significantly inhibit 76 other protein kinases tested as well as seven lipid kinases including PI3K and AKT.

KU 0063794 is a potent and selective inhibitor of mammalian target of rapamycin (mTOR); inhibits both mTORC1 and mTORC2 in vitro and in vivo.

Caratteristiche e vantaggi

This compound is featured on the PKB/Akt page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable