SAE0049

Lactic Dehydrogenase, recombinant

from human, recombinant, expressed in E. coli, aqueous solution

• Sinonimo/i: (S)-Lactate: NAD⁺ oxidoreductase, L-Lactate Dehydrogenase
• Numero CAS: 9001-60-9
• Classificazione EC (Enzyme Commission): 1.1.1.27 (BRENDA, IUBMB)
• Numero MDL: MFCD00131466
• Codice UNSPSC: 12352200
• NACRES: NA.54

Proprietà

• Origine biologica: human
• Livello qualitativo: 200
• Ricombinante: expressed in E. coli
• Stato: aqueous solution
• Condizioni di stoccaggio: (Keep container tightly closed in a dry and well-ventilated place)
• Colore: colorless
• N° accesso UniProt: K1T0A2
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... LDHA(3939)

Descrizione

Descrizione generale

Research area: Cell Signaling
The gene LDHA (L-lactate dehydrogenase A chain) is mapped to human chromosome 11p15.[1] It is a subunit of lactate dehydrogenase.[2]In particular, lactic dehydrogenase A (LDHA) is mainly found in skeletal muscle, and for that reason is known as the M subunit. This recombinant form of LDHA has a C-terminal histidine-tag.

Applicazioni

L-Lactate Dehydrogenase (LDHA) has been used in in vitro phosphoglycerate mutase 1 (PGAM1) inhibitors screening assay.[3] It has also been used in a colorimetric assay for determining lactate concentration in conditioned media.[4]

Azioni biochim/fisiol

L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD⁺ to NADH and H⁺.[5]  L-lactate dehydrogenase A chain (LDHA), an enzyme involved in pyruvate metabolism,[6] LDH is responsible for the conversion of pyruvate to lactate, the final step of glycolysis. It is overexpressed in various cancers.[6][7] LDHA regulates the microenvironment of developing tumors by the hypoxia-inducible factor (HIF)-signaling pathway.[7] LDHA aids in the NAD⁺ regeneration during the β-oxidation of fatty acid.[8] LDHA (L-lactate dehydrogenase A chain) is responsible for the conversion of pyruvate to lactate, the final step of glycolysis. It is overexpressed in various cancers. In cancer cells, HIF-1a (hypoxia-inducible factor) induces the expression of LDHA, which helps in maintaining glycolysis in cells.[2]

L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD⁺ to NADH and H⁺.

Definizione di unità

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Stato fisico

Buffered aqueous solution with Hepes (pH 7.5), NaCl and glycerol.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable