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S6322

Sigma-Aldrich

Sistema adiuvante Sigma®

oil

Sinonimo/i:

Adiuvante Sigma, Sistema adiuvante

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.46
Prezzi e disponibilità al momento non sono disponibili

Stato

oil

Livello qualitativo

300

Temperatura di conservazione

2-8°C

Applicazioni

Test per adiuvanti: il titolo EIA per i topi del test è almeno 2 volte superiore di quello dei topi di controllo.

Azioni biochim/fisiol

Sigma Adjuvant System è un′emulsione olio in acqua stabile che può essere utilizzata in alternativa alle classiche emulsioni acqua in olio di Freund. È un adiuvante derivato da componenti della parete cellulare di batterie e micobatteri che forniscono un potente stimolo al sistema immunitario.

Stato fisico

Ogni flacone contiene 0,5 mg di monofosforil-lipide A (endotossina detossificata) da Salmonella minnesota e 0,5 mg di trealosio dicorinomicolato sintetico in 2% di olio (squalene) -Tween® 80-acqua.

Note legali

Sigma Adjuvant System is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Esclusione di responsabilità

Salvo diversa indicazione nel nostro catalogo o in altra documentazione fornita dall′azienda insieme al prodotto, i nostri prodotti sono destinati esclusivamente a scopi di ricerca e non devono essere utilizzati per altre finalità, inclusi a titolo esemplificativo ma non esaustivo, fini commerciali non autorizzati, applicazioni diagnostiche in vitro, usi terapeutici ex vivo o in vivo o qualsiasi altro tipo di assunzione o applicazione rivolta agli uomini o agli animali.

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08168

Timestrip Plus™ 8 °C

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H304

Classi di pericolo

Asp. Tox. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificati d'analisi (COA)

Lot/Batch Number
0000407553
0000407553
0000329924
0000235360
0000235360

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Development of monoclonal antibodies specific for Ricinus agglutinins
Brandon DL and Hernlem BJ
Food and agricultural immunology, 20(1), 11-22 (2009)
Déirdre B Ní Eidhin et al.
FEMS immunology and medical microbiology, 52(2), 207-218 (2007-12-21)
Clostridium difficile is the leading cause of infectious antibiotic-associated diarrhoea, particularly among the elderly. Its surface-layer protein (SLP) was tested as a vaccine component in a series of immunization and challenge experiments with Golden Syrian hamsters, combined with different systemic
Christina Schellenbacher et al.
Journal of virology, 83(19), 10085-10095 (2009-07-31)
The amino (N) terminus of the human papillomavirus (HPV) minor capsid protein L2 can induce low-titer, cross-neutralizing antibodies. The aim of this study was to improve immunogenicity of L2 peptides by surface display on highly ordered, self-assembled virus-like particles (VLP)
Nadja Thönes et al.
Journal of virology, 82(11), 5472-5485 (2008-04-04)
Capsomeres are considered to be an alternative to viruslike particle (VLP)-based vaccines as they can be produced in prokaryotic expression systems. So far, no detailed side-by-side comparison of VLPs and capsomeres has been performed. In the present study, we immunized
Avi-Hai Hovav et al.
Infection and immunity, 73(1), 250-257 (2004-12-25)
In this study, we examined the immunogenicity and protective efficacy of six immunodominant Mycobacterium tuberculosis recombinant antigens (85B, 38kDa, ESAT-6, CFP21, Mtb8.4, and 16kDa) in a multivalent vaccine preparation (6Ag). Gamma interferon (IFN-gamma) and monophosphoryl lipid A-trehalose dicorynomycolate (Ribi) adjuvant
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Articoli

An Introduction to Antibodies: Antigens, Epitopes and Antibodies

Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.

Contenuto correlato

Antibody Support Reagents

Access high-quality reagents for antibody workflows, including adjuvants, blocking reagents, conjugation kits, and more.

Questions

1–8 of 8 Questions  

  1. Is the adjuvant ready to use? I don't see any mention in the datasheet on how to prepare the Antigen:adjuvant mixture. Can you help with that? Thank you.

    1 answer

    1. This product requires dilution with saline prior to use. This dilution can be done with or without the addition of the antigen. See below for a brief protocol.

      Prior to antigen addition, warm contents of vial to 40-45 °C. Add 2.0 mL of sterile saline containing the desired amount of antigen.
      The final emulsion will contain a concentration of 2% oil.
      1. Inject antigen-saline solution (2 mL) directly into the vial through the rubber stopper using a syringe fitted with a 20 or 21-gauge needle (leave the cap seal in place).
      2. Vortex the vial vigorously for 2 to 3 minutes to form emulsion. Invert the vial and vortex for 1 minute to ensure reconstitution of any product adhering to the stopper.
      3. If the entire contents of the vial will not be used initially, reconstitute with 1 ml saline without antigen. This emulsion can be stored at 2-8 °C for up to 60 days. Do Not freeze.
      To use, mix aliquots 1:1 with antigen in saline.
      4. Prior to inoculation, warm the vial to 37 °C and vortex briefly.

      For full details on injection protocols and boosting, please navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquiries" under the Products Section with all the required information so that a member of the Technical Service team can reach out to assist further. Thank you.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. When using the Sigma Adjuvant System® reagent, Product No. S6322, how do the components MPL and TDM work as an adjuvant?

    1 answer

    1. MPL (monophosphoryl lipid A) will bind to CD14 on the cell surface similar to LPS.  TDM (trehalose dicorynomycolate) is an analog of one of the components of the tubercle bacillus. Both of these components act like a superantigen and activate the immune system nonspecifically.  This allows for a greater response to the immunogen delivered in this adjuvant.

      Helpful?

  4. Can you reconstitute and store the Sigma Adjuvant System® reagent (Product No. S6322) if not using the entire vial right away?

    1 answer

    1. If the entire contents of the vial will not be used initially, reconstitute with 1 ml saline without antigen.  This emulsion can then be stored at 2-8 °C for up to 60 days.  Do Not Freeze. To use, mix aliquots 1:1 with antigen in saline. Prior to inoculation, warm the vial to 37 °C and vortex briefly.  This and other information can be found on the Sigma Adjuvant System® reagent (Product No. S6322) Product Information Sheet.

      Helpful?

  5. What is used to solubilize Product No. S6322, Sigma Adjuvant System® reagent?

    1 answer

    1. The Sigma Adjuvant System® reagent (Product No. S6322) can be solubilized in a sterile saline solution containing the desired amount of antigen to a final volume of 2 ml.  For more details, please see the S6322 Product Information Sheet.

      Helpful?

  6. Is the Sigma Adjuvant System®, Product No S6322, the same as the RIBI adjuvant?

    1 answer

    1. Yes, the Sigma Adjuvant System®, Product No S6322, is also called RIBI adjuvant.

      Helpful?

  7. Has Sigma Adjuvant System®, Product S6322, been used to induce EAE (Experimental autoimmune encephalomyelitis)?

    1 answer

    1. Sigma Adjuvant System®, Product S6322 is a replacement for MPT+TDM adjuvant (Ribi, Product M6536) that was discontinued by the supplier Corixa.  Although we have not tested Product S6322 for induction of Experimental autoimmune encephalomyelitis (EAE), the following reference cites the use of MBP in Ribi adjuvant for induction of acute EAE.The Journal of ImmunologyOctober 15, 2005, 175(8): 5077-5086

      Helpful?

  8. When using the Sigma Adjuvant System®, Product No. S6322, how is the antigenic response boosted?

    1 answer

    1. With the Sigma Adjuvant System® (Product No. S6322) with mice, rabbits, rats and guinea pigs; boost on day 21 or 28, bleed 10 - 14 days after booster injection. Boost every 3 - 4 weeks thereafter. For goats; boost every four weeks. Use the same protocol for each injection.  For more information please see the Product Information Sheet for Product No. S6322.

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