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RAB0010

Angiotensin II EIA Kit

for serum, plasma, culture supernatant and cell lysates

Sinonimo/i:

Angiotensinogen, Serpin A8

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1 kit
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CHF 531.00

Informazioni su questo articolo

NACRES:
NA.32
UNSPSC Code:
12352204

CHF 531.00


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species reactivity

mouse, human, rat

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

input

sample type cell lysate
sample type culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 2.62 pg/mL
standard curve range: 0.1-1000 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... AGT(183)

General description

The Angiotensin II Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Angiotensin II peptide based on the principle of Competitive Enzyme Immunoassay. EIA-ANGII-1 can theoretically detect all active angiotensins, including ANGI, ANGII, ANGIII and ANGIV. However, it does not detect inactive angiotensinogen.

Immunogen

Angiotensin II, synthetic peptide

Application

Angiotensin II EIA Kit has been used for quantitative estimation of serum angiotensin-II.[1]
For research use only. Not for use in diagnostic procedures.
Angiotensin II EIA Kit has been used for analyzing circulating and serum levels of angiotensin II (Ang II) in mice and rats respectively.[1]
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Study on in vitro model shows that mechanical stretch induced Ang II release plays a vital role in mediating stretch-induced hypertrophic response. Ang II is implicated in maintaining blood pressure and has an important role in hypertension and in the pathophysiology of cardiovascular disorders. Ang II also has an essential role in growth induction, cell migration and mitosis of vascular smooth muscle cells. It enhances the production of collagen type I and III in fibroblasts, that results in thickening of the vascular wall and myocardium, and fibrosis. Variation in Ang II gene increases the risk of myocardial infarction, hypertension and left ventricular hypertrophy. Ang II expression is associated with heart failure, cardiac remodeling, diabetes, and the proliferative and inflammatory responses to arterial injury. The pharmacological agents such as angiotensin II-converting enzyme inhibitors or angiotensin II receptor antagonists can be used for treating Ang II-induced hypertension and congestive heart failure.

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RAB1021RAB0385RAB0006
detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

species reactivity

mouse, human, rat

species reactivity

human

species reactivity

mouse, human, rat

species reactivity

rat, mouse, human

Gene Information

human ... AGT(183)

Gene Information

human ... AGT(183)

Gene Information

human ... NPPA(4878)

Gene Information

human ... PNPLA3(80339)

input

sample type cell lysate
sample type culture supernatant(s)
sample type serum
sample type plasma

input

sample type serum sample(s)
sample type cell culture supernatant(s)
sample type plasma

input

sample type serum
sample type cell lysate
sample type plasma
sample type culture supernatant(s)

input

sample type cell lysate
sample type plasma
sample type culture supernatant(s)
sample type serum

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 2.62 pg/mL
standard curve range: 0.1-1000 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1.22 ng/ml

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.02 pg/mL
standard curve range: 0.1-1000 pg/mL

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 21 pg/mL
standard curve range: 0.1-1000 ng/mL


I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS & Determinazione del prezzo

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Classe di stoccaggio

10 - Combustible liquids



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Questions

  1. What is the expected response for the positive control in the Human Angiotensin II EIA (RAB0010)?

    1 answer
    1. The Positive Control functions as a system control to verify the functionality of the kit components. The resulting OD will not be utilized in any calculations. Any signal within the standard curve range that demonstrates competition in the assay indicates that the assay is performing as anticipated.

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