R1028
Restorase® DNA Polymerase with 10× Reaction Buffer
Enzyme blend for PCR amplification of damaged DNA
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About This Item
Codice UNSPSC:
12352202
NACRES:
NA.55
Prodotti consigliati
Forma fisica
liquid
impiego
sufficient for 50 reactions
Caratteristiche
Long & Accurate PCR
dNTPs included: no
hotstart: no
Concentrazione
2.5 units/μL
tecniche
PCR: suitable
Colore
colorless
input
purified DNA
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Descrizione generale
Restorase DNA Polymerase with 10× Reaction Buffer combines Sigma′s long and accurate enzyme technology with a small amount of DNA repair enzyme. The optimized blend will initiate the repair and further amplification of damaged DNA templates greater than 800 bp. Restorase has also been shown to increase yield on undamaged DNA templates.
Applicazioni
Restorase® DNA Polymerase with 10× Reaction Buffer has been used in DNA repair.
Azioni biochim/fisiol
DNA templates are often damaged on exposure to heat, acids, alkylating agents, and light. These damaged templates result in inefficient or failed DNA amplification by polymerase chain reaction (PCR). Restorase® DNA Polymerase repairs the damaged sites on the DNA templates and initiates subsequent template copying. Restorase treatment of the DNA depends on the level of template damage.
Caratteristiche e vantaggi
- Reliable amplification of damaged DNA
- When thermostable polymerases fail, this enzyme amplifies the sequence efficiently
- Efficient amplification of sequences in multiplex reactions
- Increased yield and amplicon specificity
- Amplifies a broad spectrum of amplicon sizes varying from 200 bp to 20 kb
- Can repair 3′ bungs, nicks, and abasic sites
Confezionamento
The enzyme is provided with an optimized 10× reaction buffer provided as 1 vial/250 units.
Altre note
Learn more about our offering of specialty enzymes at www.sigma-aldrich.com/specialtyenzymes.
View more detailed information on Restorase DNA Polymerase at www.sigma-aldrich.com/restorase.
Note legali
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Restorase is a registered trademark of Merck KGaA, Darmstadt, Germany
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Aquatic Chronic 3
Codice della classe di stoccaggio
10 - Combustible liquids
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
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Innis, M.A., et al.
PCR Protocols: A Guide to Methods and Applications (1990)
Articoli
Restorase® was developed for researchers unable to achieve amplification of damaged DNA templates when using other commercially available DNA polymerases.
Protocolli
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
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