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P6248

Sigma-Aldrich

Anti-Parkin antibody, Mouse monoclonal

clone PRK8, purified from hybridoma cell culture

Sinonimo/i:

Anti-AR-JP, Anti-LPRS2, Anti-PARK2, Anti-PDJ

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About This Item

Numero MDL:
MFCD02263896
Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Coniugato

unconjugated

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

PRK8, monoclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

mouse, rat, hamster, human

Concentrazione

~2 mg/mL

tecniche

microarray: suitable
western blot: 0.25-0.5 μg/mL using using rat brain cytosolic S1 extract

Isotipo

IgG2b

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... PARK2(5071)
mouse ... Park2(50873)
rat ... Park2(56816)

Descrizione generale

Monoclonal Anti- Parkin (mouse IgG2b isotype) is derived from the hybridoma PRK8 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human Parkin. Parkin is an ubiquitin-protein ligase expresses in many tissues such as brain, testis, skeletal muscle and heart. The parkin gene encodes a protein of 465 amino acid residues with moderate similarity to ubiquitin at the amino-terminus and a ring in between ring fingers (IBR)-ring-finger motif at the carboxyl-terminus. The gene has 12 exons.
Parkin is an ubiquitin-protein ligase expresses in many tissues such as brain, testis, skeletal muscle and heart. It is a RING-type E3 ubiquitin-protein ligase that facilitates the ubiquitination of misfolded proteins and protects from neurotoxic effect caused by these proteins. Monoclonal anti-parkin antibody can be used in microarray and immunoprecipitation assays. It can also be used in western blotting. Mouse anti-parkin antibody reacts specifically with parkin (50kD approx) of rat, hamster, mouse and human.

Immunogeno

recombinant human Parkin.

Applicazioni

Monoclonal anti-parkin antibody has been used in:
  • Enzyme linked immunosorbent assay (ELISA)
  • Immunoblotting
  • Immunoprecipitation[63}
  • Immunocytochemistry
  • to label parkin in pull-down assay

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificati d'analisi (COA)

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Victoriane Peugnet et al.
Antioxidants (Basel, Switzerland), 11(4) (2022-04-24)
Heart failure, mostly associated with cardiac hypertrophy, is a major cause of illness and death. Oxidative stress causes accumulation of reactive oxygen species (ROS), leading to mitochondrial dysfunction, suggesting that mitochondria-targeted therapies could be effective in this context. The purpose
Genomic organization and expression of parkin in Drosophila melanogaster
Bae JY, et al.
Experimental & Molecular Medicine, 35(5), 393-393 (2003)
Parkin regulates kainate receptors by interacting with the GluK2 subunit
Maraschi AM, et al,
Nature Communications, 5(46) (2014)
Koji Yamano et al.
The Journal of biological chemistry, 290(42), 25199-25211 (2015-08-12)
Damaged mitochondria are eliminated through autophagy machinery. A cytosolic E3 ubiquitin ligase Parkin, a gene product mutated in familial Parkinsonism, is essential for this pathway. Recent progress has revealed that phosphorylation of both Parkin and ubiquitin at Ser(65) by PINK1
Takaya Ishihara et al.
Molecular and cellular biology, 35(1), 211-223 (2014-10-29)
Mitochondria are dynamic organelles, and their fusion and fission regulate cellular signaling, development, and mitochondrial homeostasis, including mitochondrial DNA (mtDNA) distribution. Cardiac myocytes have a specialized cytoplasmic structure where large mitochondria are aligned into tightly packed myofibril bundles; however, recent
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