P3818

Protein Disulfide Isomerase from bovine liver

≥100 units/mg protein, lyophilized powder

• Sinonimo/i: PDI
• Numero CAS: 37318-49-3
• Classificazione EC (Enzyme Commission): 5.3.4.1 (BRENDA, IUBMB)
• Numero MDL: MFCD00163364
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Stato: lyophilized powder
• Livello qualitativo: 200
• Attività specifica: ≥100 units/mg protein
• PM: 107 kDa
• Composizione: Protein, ~10% Lowry
• Temperatura di conservazione: −20°C
• Stringa SMILE: [S](=O)(=O)(ON)c1c(cc(c(c1)C(=O)O)Cl)Cl
• InChI: 1S/C7H5Cl2NO5S/c8-4-2-5(9)6(16(13,14)15-10)1-3(4)7(11)12/h1-2H,10H2,(H,11,12); DHUYKLYJBKXDBM-UHFFFAOYSA-N

Descrizione

Descrizione generale

Protein disulfide isomerase (PDI) from bovine liver is a homodimer with a molecular weight of 107 kDa with the monomer corresponding to 57 kDa. The isoelectric point (pI) is approximately 4.2.[1] The enzyme is a glycoprotein with 12% total carbohydrate content comprising of mannose, galactose, N-acetyl neuraminic acid (NANA) and 2-acetamido-2-deoxyglucose.[2] PDI is an ubiquitous redox chaperone enzyme. It belongs to the, thioredoxin superfamily and is high conserved.[3]

Applicazioni

Protein Disulfide Isomerase from bovine liver has been used:

• to study the functional role of PDI in parasite infection and the interaction between macrophage PDI and L. chagasi[3]
• in the in vitro translation reaction for the generation of disulfide bonds[4]
• in insulin-disulfide reduction assay and peptide binding assay[5]
• as a positive control in thiol-disulfide oxidoreductase activity assay[6]

Azioni biochim/fisiol

Protein Disulfide Isomerase (PDI) is mainly located in the endoplasmic reticulum (ER), where it assists in protein-folding and thiol-disulfide exchanges.[4] It aids protein refolding in vitro allowing recombinant proteins to achieve their native state.[7]

Protein Disulfide Isomerase(PDI) has the C-terminal ER retention sequence Lys-Asp-Glu-Leu. It has active, intracellular traffic to different cell compartments. PDI supports internalization of Chlamydia, cholera and diphtheria toxins in some hosts. PDI is required for Sindbis virus infection and aids in reducing HIV gp120 protein thiols. PDI facilitates formation of the correct disulfide bonds by promoting rapid reshuffling of disulfide pairings.[8]

Confezionamento

Package size based on protein content.

Proprietà fisiche

Protein Disulfide Isomerase (PDI) from bovine liver is a homodimer with a molecular weight of 107 kDa (gel filtration) and the molecular weight of the monomer has been reported at 57 kDA (SDS-PAGE). The enzyme is a glycoprotein with 12% total carbohydrate content, composed of 4.6% mannose, 2.5% galactose, 1.4% NANA, and 3.5% 2-acetamido-2-deoxyglucose.

Definizione di unità

One unit cause a change in A₆₅₀ of 0.01 per min of a 1.0 mg/mL solution of insulin in the presence of dithiothreitol at pH 7.5 at 25 °C.

Stato fisico

Lyophilized powder containing potassium phosphate buffer salts and stabilizer.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)