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P0001

Sigma-Aldrich

Phosphatase Inhibitor Cocktail

ReadyShield® ready-to-use solution, for the inhibition of Serine/Threonine Protein Phosphatases and L-Isozymes of Alkaline Phosphatase

Sinonimo/i:

Phosphatase inhibitor cocktail

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.54

product name

ReadyShield® Phosphatase Inhibitor Cocktail, For Serine/Threonine Protein Phosphatases and L-Isozymes of Alkaline Phosphatase

Livello qualitativo

Forma fisica

ready-to-use solution

Confezionamento

pkg of 1 mL (in amber polypropylene vial)
pkg of 5 mL (in amber polypropylene vial)

Temperatura di conservazione

−20°C

Descrizione generale

This phosphatase Inhibitor Cocktail contains inhibitors with a broad specificity for L-isozymes of alkaline phosphatases, and serine/ threonine protein phosphatases. Phosphatase Inhibitor Cocktail has been tested on extracts from various animal tissues such as human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells.

This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation.
ReadyShield® P0001 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P0044.

Caratteristiche e vantaggi

  • Does not freeze in storage for quick, convenient use.
  • Specific for Serine/Threonine Protein Phosphatases and L-Isozymes of Alkaline Phosphatase.
  • Suggested as 100 X solution.
  • Reclosable screw-top vials.
  • Can be stored in cooler for approx. 1 month or room temperature for a week.

Nota sulla preparazione

This cocktail is supplied as a clear solution in dimethyl sulfoxide. It is recommended to dilute the cocktail at 1 mL of cocktail per 500 mg of protein extracted from tissue or cells. Often a final concentration of 1% (v/v) is used, which will inhibit phosphatase activities found in the 27,000 x g supernatant from human placenta and bovine liver, and in the 100,000 x g supernatant from A431, CHO, and U937 extracts.

Altre note

For life science research only. Not for use in diagnostic procedures.

Note legali

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Articoli

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.