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MRN70

Sigma-Aldrich

GenElute™ mRNA Miniprep Kit

sufficient for 70 purifications

Sinonimo/i:

GenElute™ mRNA Kit, Gen Elute

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About This Item

Codice UNSPSC:
41105501
NACRES:
NA.52

impiego

sufficient for 70 purifications

tecniche

RNA purification: suitable

Temperatura di conservazione

15-25°C

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Descrizione generale

Procedures such as cDNA synthesis, expression profiling and others require separation of mRNA from the vastly more abundant rRNA and tRNA. The GenElute mRNA kits provide convenient procedures for isolating polyadenylated mRNA from previously prepared total RNA or directly from mammalian cells and tissues. For direct mRNA preparation, cells or tissues are disrupted with SDS/proteinase K digestion to release RNA and eliminate RNases. Both kit types use oligo dT30 covalently linked to 1 μm polystyrene beads to capture polyadenylated mRNA by hybridization. The polystyrene beads remain suspended during hybridization, eliminating the need for mixing or rocking, as is common for cellulose or magnetic particles. Polystyrene was also chosen because oligo(dT) polystyrene beads yield cleaner mRNA with fewer stringent washing steps than does the more commonly used oligo(dT) cellulose (2 or 3 wash steps versus 10 or more). With the GenElute kits, mRNA-bead complexes are washed on a microcentrifuge spin filter, and eluted into 10 mM Tris-HCL, pH 7.5. mRNA prepared with either kit is suitable for a variety of downstream applications such as Northern blotting, expression array or chip hybridizations and cDNA synthesis and library construction.

Applicazioni

GenElute™ mRNA Miniprep Kit has been used to purify RNA from total RNA and to isolate RNA.
The purified mRNA is ready for Northern analysis, reverse transcription and PCR, labeling for arrays, and other common applications.

Caratteristiche e vantaggi

  • Quick and convenient reagent for use in the simultaneous isolation of RNA, DNA and protein
  • Performs well with large or small amounts of tissue or cells and many samples can be simultaneously extracted
  • One of the most effective methods for isolating total RNA. Purifications can be completed in only one hour starting with fresh tissue or cells

Altre note

For additional information, please see www.sigma-aldrich.com/mrna.

Note legali

GenElute is a trademark of Sigma-Aldrich Co. LLC

Codice della classe di stoccaggio

10 - Combustible liquids

Certificati d'analisi (COA)

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Transcriptome-wide mapping of N(6)-methyladenosine by m(6)A-seq based on immunocapturing and massively parallel sequencing.
Dominissini D
Nature Protocols, 8(1), 176-189 (2013)
The nuclear retention signal of HPV16 L2 protein is essential for incoming viral genome to transverse the trans-Golgi network.
DiGiuseppe S, Bienkowska-Haba M, Hilbig L, et al.
Virology, 458-459, 93-105 (2014)
K Oishi et al.
Journal of thrombosis and haemostasis : JTH, 4(7), 1566-1574 (2006-07-15)
Diabetes is associated with an excess risk of cardiac events, and one risk factor for infarction is an elevated level of plasminogen activator inhibitor-1 (PAI-1). To evaluate whether the glucocorticoid hormones are involved in the diabetes-induced PAI-1 production, we examined
Enjie Li et al.
eLife, 11 (2022-05-04)
Methyltransferase-like 3 (METTL3) and N6-methyladenosine (m6A) are involved in many types of biological and pathological processes, including DNA repair. However, the function and mechanism of METTL3 in DNA repair and chemotherapeutic response remain largely unknown. In present study, we identified
Adrenal gland-dependent augmentation of plasminogen activator inhibitor-1 expression in streptozotocin-induced diabetic mice.
Oishi K
Journal of Thrombosis and Haemostasis, 4(7), 1566-1574 (2006)
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Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

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