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Sigma-Aldrich

GenElute™ Direct mRNA Miniprep Kits

sufficient for 10 purifications

Sinonimo/i:

GenElute™ Direct mRNA Miniprep Kit, GenElute™ mRNA Kit, Gen Elute

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About This Item

Codice UNSPSC:
41105501
NACRES:
NA.52

impiego

sufficient for 10 purifications

tecniche

RNA purification: suitable

Temperatura di conservazione

15-25°C

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Descrizione generale

The GenElute™ Direct mRNA Miniprep kit provides a convenient format to isolate polyadenylated mRNA directly from mammalian cells and tissues. The direct mRNA isolation procedure is based on that of Badley. Up to 107 mammalian cells or 40 mg tissue are lysed and homogenized, either with the filtration columns provided or with
a mechanical homogenizer. RNase is eliminated during a 10 minute proteinase K digestion. Sodium chloride is added, and polyadenylated RNA is captured on oligo(dT) polystyrene beads during a 10 minute incubation. For further enrichment, RNA may be released from the beads into fresh lysis solution and recaptured with the original beads. After 3 washes in a spin column, purified mRNA is eluted in 100 μl of 10 mM Tris-HCl, pH 7.4.

Applicazioni

The GenElute Direct Kit mRNA kit provides convenient procedures for isolating polyadenylated mRNA from previously prepared total RNA or directly from mammalian cells and tissues.

Caratteristiche e vantaggi

  • mRNA captured on oligo(dT) polystyrene beads in 10 minutes, with no mixing or rocking (Fig. 1)
  • Poly (A)+ mRNA isolated from total RNA in 40 minutes (Fig. 2) or 60 minutes directly from cells and tissues (Fig. 3)
  • Oligo(dT) polystyrene beads require fewer wash steps

Altre note

Use for isolating mRNA directly from mammalian cells or tissues.
For additional information, please see www.sigma-aldrich.com/mrna.

Note legali

GenElute is a trademark of Sigma-Aldrich Co. LLC

Solo come componenti del kit

N° Catalogo
Descrizione
  • Elution solution 1.5 mL
  • Filtration columns with tubes 10 ea
  • Lysis solution 20 mL
  • 5 M NaCl 1.5 mL
  • Oligo(dT)-polystyrene beads .3 mL
  • Proteinase K 5 mg
  • 40% Glycerol solution .6 mL
  • Spin columns with tubes 10 ea
  • Collection tube 10 ea
Vedi tutto (9)

Avvertenze

Danger

Indicazioni di pericolo

H290,H315,H319,H334,H335

Classi di pericolo

Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Protocolli

Reverse Transcription Protocol

Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.

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