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MAK322

Sigma-Aldrich

Collagen Assay Kit

sufficient for 100 fluorometric tests

Sinonimo/i:

Collagen Quantification Kit

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

impiego

sufficient for 100 fluorometric tests

input

cell
serum
plasma
solution(s) (collagen)

applicazioni

cosmetics
food and beverages

Metodo di rivelazione

fluorometric

Malattie correlate

rheumatological diseases; dermatological diseases; gastrointestinal diseases; pulmonary disorders; cancer

Temperatura di conservazione

−20°C

Categorie correlate

Descrizione generale

Collagen is the key structural protein of connective tissue and the most abundant protein in mammals. It occurs in many different types and forms with Types I -V being the most common. Aside from the crucial role it plays in the body, it has numerous medical applications such as its use in reconstructive surgery including bone and skin grafts. It is also commonly used in cosmetics due to its anti-aging and skin healing properties.

Applicazioni

The Collagen Assay Kit may be used for:
  • Bone Disorder Research
  • Dermatological Research
  • Wound Healing and Tissue Repair Research


Caratteristiche e vantaggi

Enhanced Detection Range: Detect Collagen levels accurately across a wide range, from 2 µg/mL to 50 µg/mL, using only 20 μL of sample in a 96-well plate setup, ensuring highly sensitive analysis with minimal sample requirement.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Compatibilità

Suitable for biological samples (e.g. plasma, serum, cells) and cosmetic samples (e.g. collagen solution)

Principio

Assay methods available for quantifying collagen currently range from needing extensive hydrolysis procedures with acids and bases to using expensive antibodies and complicated protocols. The collagen assay kit delivers a very simple, safe (non-radioactive), and sensitive method to quantify collagen in samples. In the first step of this procedure, collagen in the sample is enzymatically digested into peptides. Subsequently, the N-terminal glycine containing peptides react with the dye reagent to form a fluorescent complex. The fluorescence intensity of this product, measured at λex/em = 375/465 nm, is directly proportional to collagen concentration in the sample.

Altre note

For additional information on our range of Biochemicals, please complete this form.

Pittogrammi

Health hazard

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Repr. 1B - Resp. Sens. 1

Codice della classe di stoccaggio

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects


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