The protocol is included in the product datasheet for this item. Please see the link below to review this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/270/922/mak196bul.pdf
MAK196
Malate Dehydrogenase Assay Kit
Sufficient for 100 Colorimetric tests
Sinonimo/i:
MDH Activity Assay Kit
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CHF 561.00
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1 KIT
CHF 561.00
About This Item
Codice UNSPSC:
12161503
NACRES:
NA.84
Prodotti consigliati
Metodo di rivelazione
colorimetric
Malattie correlate
neurological disorders
Temperatura di conservazione
−20°C
Informazioni sul gene
human ... MDH1(4190) , MDH1B(130752) , MDH2(4191)
mouse ... MDH1(17449) , MDH1B(76668) , MDH2(17448)
rat ... MDH1(24551) , MDH1B(316444) , MDH2(81829)
Categorie correlate
Descrizione generale
Malate dehydrogenase (MDH; EC 1.1.1.37) is an NAD oxidoreductase that catalyzes the NAD/NADH-dependent interconversion of malate and oxalacetate. This reaction is critical to maintain the malate/aspartate shuttle across the mitochondrial membrane and Krebs tricarboxylic acid cycle in the mitochondrial matrix. Increased MDH activity has been observed in neurodegenerative diseases such as Alzheimer′s disease.
Applicazioni
Compatibilità
Suitable for the detection of Malate Dehydrogenase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.
Principio
The Malate Dehydrogenase Assay Kit provides a simple and sensitive procedure for measuring MDH activity in a variety of tissues, cell cultures, and isolated mitochondria. MDH activity is determined by generating a product with absorbance at 450 nm proportional to the enzymatic activity present. One unit of MDH is the amount of enzyme that generates 1.0 μmole of NADH per minute at 37 °C and pH 9.5.
Solo come componenti del kit
N° Catalogo
Descrizione
- MDH Assay Buffer
- MDH Substrate
- MDH Enzyme Mix
- MDH Developer
- NADH Standard
- MDH Positive Control
Sostituito da
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Danger
Indicazioni di pericolo
Classi di pericolo
Eye Dam. 1 - Skin Corr. 1B
Codice della classe di stoccaggio
8A - Combustible corrosive hazardous materials
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Protocolli
Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.
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Is there a protocol associated with the kit? This general protocol doesn't use MDH developer: https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/_enzymatic-assay-of-malic-dehydrogenase
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