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MAK333

Sigma-Aldrich

Citrate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Sinonimo/i:

Citric Acid Test Kit

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

impiego

sufficient for 100 colorimetric or fluorometric tests

input

culture(s)
plasma
tissue
serum
urine

Metodo di rivelazione

colorimetric
fluorometric

Malattie correlate

cancer

Temperatura di conservazione

−20°C

Descrizione generale

Citrate is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. The Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays.

Applicazioni

The Citrate Assay Kit may be used for:
  • Cancer Research
  • Drug Discovery
  • Metabolic Disorders Research

Caratteristiche e vantaggi

Efficient and Precise: Detect Citrate levels ranging from 4 to 400 μM (colorimetric) and 0.5 to 40 μM (fluorimetric) in a 15 minute room temperature reaction, providing both convenience and sensitivity.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Compatibilità

Suitable for citrate determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)

Principio

In this kit, citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays.

Altre note

For additional information on our range of Biochemicals, please complete this form.

Codice della classe di stoccaggio

10 - Combustible liquids


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