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MAK014

Sigma-Aldrich

Glucose-6-Phosphate Assay Kit

sufficient for 100 colorimetric tests

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About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

impiego

sufficient for 100 colorimetric tests

Metodo di rivelazione

colorimetric

Malattie correlate

hematological disorder

Temperatura di conservazione

−20°C

Descrizione generale

Glucose 6-phosphate (G6P) is a key metabolic intermediate that enters either metabolic pathways or storage. G6P is generated when glucose is phosphorylated by hexokinase or glucokinase or by the conversion of glucose-1-phosphate by phosphoglucomutase during glycogenolysis. G6P lies at the beginning of both glycolysis and the pentose phosphate pathways. It also can be stored as glycogen when blood glucose levels are high.[1]

Applicazioni

Glucose-6-phosphate assay kit has been used in:

  • glucose 6-phosphate (G6P) assay[2]
  • quantification of glucose-6-phosphate (G6P)[3][4][5] and fructose-6-phosphate (F6P)[3]
  • metabolite analysis[6]

Compatibilità

Suitable for quantifying Glucose-6-Phosphate in a variety of samples.

Principio

G6P is utilized by Glucose-6-phosphate dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. G6P is determined by an enzyme assay, which results in a colorimetric (450 nm) product, proportional to the G6P present. Typical sensitivities of detection are between 1-30 nmoles.

Sostituito da

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazardCorrosion
GHS08,GHS05

Avvertenze

Danger

Indicazioni di pericolo

H314,H334

Classi di pericolo

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

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Certificati d'analisi (COA)

Lot/Batch Number
8J08K06570
8D21K06570
7K07K06570
7D12K06570
6H06K06570

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MiR-182 promotes glucose metabolism by upregulating hypoxia-inducible factor 1a in NSCLC cells
Mingliang W, et al.
Biochemical and Biophysical Research Communications, 504(2), 400-405 (2018)
Saixiang Feng et al.
Veterinary research, 51(1), 97-97 (2020-08-02)
Lipooligosaccharides (LOSs) are virulence determinants of Glaesserella parasuis, a pathogen of the respiratory tract of pigs. We previously reported that disruption of the galU or galE gene in G. parasuis results in increased sensitivity to porcine serum, indicating that the
Renal Disease in Type I Glycogen Storage Disease
Richard P L, et al.
Genetic Diseases of the Kidney (2009)
Guillermo Martín-Gutiérrez et al.
Antibiotics (Basel, Switzerland), 9(11) (2020-11-18)
Mutations that confer low-level fosfomycin resistance (LLFR) but not clinical resistance in Escherichia coli are increasingly reported. LLFR strains can become clinically resistant under urinary tract physiological conditions or may act as gateways for highly resistant subpopulations by the selection
Forkhead Box O6 (FoxO6) Depletion Attenuates Hepatic Gluconeogenesis and Protects against Fat-induced Glucose Disorder in Mice.
Virtu C N, et al.
The Journal of Biological Chemistry, 290, 15581-15594 (2015)
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Aerobic Glycolysis and the Warburg Effect

Warburg effect enhances glucose to lactate conversion in tumor cells, regardless of oxygen levels; impacting cancer metabolism since 1924.

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