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MAK013

Sigma-Aldrich

Glucose and Sucrose Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Sinonimo/i:

Glucose and Sucrose Colorimetric/Fluorometric Assay Kit

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About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

impiego

sufficient for 100 colorimetric or fluorometric tests

applicazioni

cosmetics
food and beverages

Metodo di rivelazione

colorimetric
fluorometric

Malattie correlate

endocrinological disorders, diabetes

Temperatura di conservazione

−20°C

Descrizione generale

Sucrose is a disaccharide that is hydrolyzed to glucose and fructose by invertase.[1] Glucose is the primary carbohydrate utilized for energy during cellular respiration.[2] Upon cellular uptake, glucose can be shunted towards breakdown via glycolysis or polymerized for energy storage.[2]

Applicazioni

Glucose and sucrose assay kit has been used to:
  • determine sucrose,[3] aerobic glycolysis and pyruvate kinase activity[4]
  • determine protein and sugar in strawberries[5]
  • determine plasma insulin and glucose levels in mice[6]
  • analyze plasma glucose and plasma lactate[7] and to quantify glucose/sucrose.[8]

Compatibilità

Suitable for use with various biological samples including serum, plasma, other body fluids, food, and growth media.

Principio

In this assay kit, glucose is oxidized via glucose oxidase resulting in a colorimetric (570 nm)/ fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the glucose present. To measure sucrose, invertase is added to the reaction to convert the sucrose to glucose and fructose. The free glucose can be subtracted from the total glucose to give the concentration of sucrose present. This kit has a linear range of detection between 0.2-1.0 nmole of glucose for the fluorometric assay and 2-10 nmoles of glucose for the colorimetric assay.

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334,H412

Consigli di prudenza

P261 - P273 - P284 - P501

Classi di pericolo

Aquatic Chronic 3 - Resp. Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

188.6 °F - closed cup

Punto d’infiammabilità (°C)

87 °C - closed cup

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Certificati d'analisi (COA)

Lot/Batch Number
8A20K06160
8F07K06160
8K28K06160
7E12K06160
6K06K06160

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Questions

1–2 of 2 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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