M1302

M-MLV Reverse Transcriptase

Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis

• Sinonimo/i: Moloney Murine Leukemia Virus Reverse Transcriptase
• Numero CAS: 9068-38-6
• Numero MDL: MFCD00283754
• Codice UNSPSC: 12352202
• NACRES: NA.55

Proprietà

• Origine biologica: Porcine intestinal mucosa
• Livello qualitativo: 200
• Ricombinante: expressed in E. coli
• Stato: liquid
• impiego: sufficient for 200 reactions; sufficient for 250 reactions
• Caratteristiche: dNTPs included: no; hotstart: no
• Concentrazione: 200 units/μL
• tecniche: RT-PCR: suitable
• Colore: colorless
• input: purified RNA
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Moloney murine leukemia virus (M-MLV ) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues.[1] It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.

Applicazioni

M-MLV Reverse Transcriptase has been used:

• for the preparation of cDNA libraries or for first strand cDNA synthesis[2][3]
• for use in a 2-step RT-PCR assay[4][5][6]
• in quantitative realtime-polymerase chain reaction (RT-qPCR)[5]
• in reverse transcription[6]

Caratteristiche e vantaggi

• Thermostable reverse transcriptase active at 37 °C.
• Can be used to generate first strand cDNA of up to 7 kb.

Confezionamento

Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.

Definizione di unità

One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer.

Nota sulla preparazione

The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid.

Note legali

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H334
• Consigli di prudenza: P261 - P284 - P501
• Classi di pericolo: Resp. Sens. 1
• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)