Passa al contenuto
Merck
Tutte le immagini(2)

Documenti

L8754

Sigma-Aldrich

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-P, lyophilized powder

Sinonimo/i:

Phaseolus vulgaris agglutinin, PHA

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(2)

About This Item

Numero CE:
232-718-7
Codice UNSPSC:
12352202
NACRES:
NA.32

Origine biologica

Phaseolus vulgaris

Forma fisica

lyophilized powder

Potenza

≤62.5 μg per mL agglutination activity
≤10 μg/mL mitogenic activity

Impurezze

salt, free

Temperatura di conservazione

2-8°C

Cerchi prodotti simili? Visita Guida al confronto tra prodotti

Descrizione generale

PHAs are carbohydrate-binding proteins. There are several types of PHAs identified, predominantly in leguminous plants.
Phytohaemagglutinin (PHA, or phytohemagglutinin) is a lectin found in plants (esp. in legumes). It is obtained from Phaseolus vulgaris (red kidney beans) and acts as a mitogen in human leucocyte culture.

Applicazioni

It has also been used as an antigen to measure in vivo T-cell-mediated immune responsiveness.
Lectin from Phaseolus vulgaris (red kidney bean) has been used in phytohemagglutinin (PHA) assay and during assessment of cellular and humoral immunity.

Azioni biochim/fisiol

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Risultati analitici

Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Andrew Z Wang et al.
The Journal of experimental biology, 223(Pt 20) (2020-09-13)
Limited resources must be partitioned among traits that enhance fitness. Although survival-related traits often trade off with reproduction, survival-related traits themselves may trade off with each other under energy limitations. Whole-organism performance and the immune system both enhance survival, yet
Qianting Yang et al.
Thorax, 75(7), 576-583 (2020-03-24)
Biomarker-based tests for diagnosing TB currently rely on detecting Mycobacterium tuberculosis (Mtb) antigen-specific cellular responses. While this approach can detect Mtb infection, it is not efficient in diagnosing TB, especially for patients who lack aetiological evidence of the disease. We
Phytohemagglutinin content in fresh kidney bean in China
Sun Y, et al.
International journal of food properties, 22(1), 405-413 (2019)
Pheasant sexual ornaments reflect nutritional conditions during early growth
Ohlsson T, et al.
Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character. Royal Society (Great Britain), 269(1486), 21-27 (2002)
Mathieu Troïanowski et al.
Conservation biology : the journal of the Society for Conservation Biology, 31(5), 1132-1140 (2017-01-12)
During the last decade, many studies have focused on the detrimental effects of noise pollution on acoustic communication. Surprisingly, although it is known that noise exposure strongly influences health in humans, studies on wildlife remain scarce. In order to gain
Visualizza tutti i documenti correlati

Articoli

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.