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L7647

Sigma-Aldrich

Concanavalin A from Canavalia ensiformis (Jack bean)

Type VI, lyophilized powder

Sinonimo/i:

Con A

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About This Item

Numero CAS:
11028-71-0
Numero CE:
234-258-2
Numero MDL:
MFCD00071069
Codice UNSPSC:
12352202
NACRES:
NA.32

Origine biologica

Canavalia ensiformis

Tipo

Type VI

Forma fisica

lyophilized powder

Potenza

≤20 μg per mL agglutination activity

Impurezze

Carbohydrate, essentially free
 salt, essentially free

Solubilità

water: 1 % (w/v)

Temperatura di conservazione

−20°C

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Categorie correlate

Applicazioni

Concanavalin A from Canavalia ensiformis (Jack bean) has been used for developing zwitterion-immobilized molecularly imprinted polymers (ziMIPs) for protein crystallization. It has been also used in the preparation of protein loaded microgels to prevent enzymatic degradation of microgels for monitoring by confocal laser scanning microscope (CLSM).

Azioni biochim/fisiol

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Nota sulla preparazione

Highly purified by specific affinity chromatography.

Risultati analitici

Agglutination activity: Determined from serial dilutions of a 1 mg/mL solution (this activity is the lowest conc. to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C). Requires Type A Human Blood.

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H317,H334,H361

Classi di pericolo

Repr. 2 - Resp. Sens. 1 - Skin Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificati d'analisi (COA)

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