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L1412

Sigma-Aldrich

Lysing Enzymes from Trichoderma harzianum

lyophilized powder

Sinonimo/i:

Glucanex®

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About This Item

Codice UNSPSC:
12352204

Stato

lyophilized powder

Attività specifica

≥10 units/g

applicazioni

diagnostic assay manufacturing

Temperatura di conservazione

2-8°C

Applicazioni

Lysing Enzymes from Trichoderma harzianum is suitable retrieve DNA plugs from agarose gels. It has been used:
  • for protoplast isolation
  • for hydrolyzing poly(1-3)-glucose of the yeast cell wall glucan prior to yeast spheroplast transformation
  • for the lysis of Aspergillus niger vegetative cells

Azioni biochim/fisiol

Lysing Enzymes from Trichoderma harzianum is called Glucanex. It comprised of mixture of isoenzymes with β-1, 3 glucanase activity. Lysing Enzymes from Trichoderma harzianum hydrolyzes yeast cell wall oligosaccharides to glucan for protoplast preparation. Its stability is influenced by pH changes.

Qualità

Contains β-glucanase, cellulase, protease, and chitinase activities

Definizione di unità

One unit will hydrolyze casein to produce color equivalent to 1.0 micromole (181 μg) of tyrosine per minute at ph 7.5 at 37 deg C (color byfolin-ciocalteu reagent).

Note legali

A product of Novozyme Corp.
Glucanex is a registered trademark of Novozymes Corp.

Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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