The solubility of H375, Hyaluronidase from bovine testes, is 100 mg/mL in water. To achieve a higher solubility, it is advised to warm the tube at 37°C and shake it in an ultrasonic bath for a period. The stock solution can be stored at temperatures below -20°C for several months.
H3757
Ialuronidasi
Type VIII, lyophilized powder, 300-1,000 U/mg
Sinonimo/i:
Ialuronato 4-glicanoidrolasi, Ialuronoglucosaminidasi
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100 MG
CHF 108.00
10 X 100 MG
CHF 833.00
CHF 108.00
Spedizione prevista il16 aprile 2025
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Cambia visualizzazione
100 MG
CHF 108.00
10 X 100 MG
CHF 833.00
About This Item
Numero CAS:
Numero CE:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54
CHF 108.00
Spedizione prevista il16 aprile 2025
Prodotti consigliati
Origine biologica
bovine testis
Livello qualitativo
Coniugato
conjugate (Glucosaminoglycan)
Tipo
Type VIII
Stato
lyophilized powder
Attività specifica
300-1,000 U/mg
PM
~55 kDa (four subunits of 14 kDa each)
Malattie correlate
cancer
Temperatura di conservazione
−20°C
Informazioni sul gene
cow ... HYAL1(515397) , HYAL2(281838)
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Azioni biochim/fisiol
La ialuronidasi degrada lo ialuronano ed è stato scoperto che è regolata in maniera inappropriata durante la progressione del cancro.
Questi enzimi scindono casualmente i legami glicosidici della β-N-acetilesosammina-[1→4] nell′acido ialuronico, nella condroitina e nei condroitina solfati.
Definizione di unità
One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
Nota sulla preparazione
Prepared from aseptic filled solution of Type I-S.
Altre note
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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Articoli
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Protocolli
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
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What is the recommended method for reconstituting item H375, Hyaluronidase from bovine testes?
1 answer-
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Good Morning, I our lab we got this product (H3757) and we would like to know how to obtain the package insert since we are not sure how to store it or after open expiration date. Not lot number on the bottle so not sure how to look for it. Thank you
1 answer-
Hyaluronidase from bovine testes may be reconstituted at 1 mg/mL in 0.02 M sodium phosphate buffer, pH 7.0 containing 77mM sodium chloride and 0.01% bovine serum albumin. For more information please view the assay below:
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How is product H3757, Hyaluronidase from bovine testes, solubilized?
1 answer-
Product H3757, Hyaluronidase from bovine testes, may be reconstituted at 1 mg/mL in 0.02 M sodium phosphate buffer, pH 7.0 containing 77mM sodium chloride and 0.01% bovine serum albumin.
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