Passa al contenuto
Merck
Tutte le immagini(1)

Documenti fondamentali

GE17-0920-07

IMAC Sepharose™ High Performance

Cytiva 17-0920-07, pack of 100 mL

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(1)

About This Item

Codice UNSPSC:
41106500
NACRES:
NA.56
Prezzi e disponibilità al momento non sono disponibili

Confezionamento

pack of 100 mL

Produttore/marchio commerciale

Cytiva 17-0920-07

Matrice

highly cross-linked 6% agarose

Dimens. media delle particelle

34 μm

cleaning in place

2-14(Medium uncharged with metal ions.)

Intervallo di esercizio

3-12

Capacità

≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

Categorie correlate

Descrizione generale

IMAC Sepharose™ High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

Applicazioni

Conveniently charge with your metal of choice. IMAC Sepharose™ FF is supplied free of metal ions. It is charged by the user with the transition metal ion of choice (e.g. Cu2?, Zn2?, Ni2?, or Co2?); these metal ions will bind to the covalently immobilized chelating ligand on the Sepharose. Small bead size for high performance purification and high binding capacity.

Caratteristiche e vantaggi

  • For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion
  • Conveniently charge with your metal of choice
  • 34 μm bead size for high performance purification
  • High binding capacity

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Sepharose is a trademark of Cytiva

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Codice della classe di stoccaggio

3 - Flammable liquids

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documenti section.

Se ti serve aiuto, non esitare a contattarci Servizio Clienti

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

I clienti hanno visto anche

Slide 1 of 6

1 of 6

HiPrep™ IMAC Fast Flow 16/10 Cytiva 28-9365-52

GE28-9365-52

HiPrep™ IMAC Fast Flow 16/10

Ni Sepharose™ 6Fast Flow Cytiva 17-5318-01, pack of 25 mL

GE17-5318-01

Ni Sepharose™ 6Fast Flow

PHOS-Select™ Iron Affinity Gel

Sigma-Aldrich

P9740

PHOS-Select™ Iron Affinity Gel

Iminodiacetic acid Agarose saline suspension

Sigma-Aldrich

I4758

Iminodiacetic acid Agarose

HiTrap® IMAC Fast Flow Cytiva 17-0921-04, pack of 5 × 5 mL

GE17-0921-04

HiTrap® IMAC Fast Flow

Ni Sepharose™ High Performance Cytiva 17-5268-01, pack of 25 mL

GE17-5268-01

Ni Sepharose™ High Performance

Articoli

Immobilized Metal Chelate Affinity Chromatography (IMAC)

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

Purification or Removal of Proteins and Peptides

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.

Cell Lysis of Histidine-Tagged Proteins

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

Optimizing Purification of Histidine-Tagged Proteins

How to optimize purification of histidine-tagged proteins using Cytiva products.

Vedi tutto

Protocolli

Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance

Protocol for Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance

Separation with Cytiva Products

Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

Vedi tutto

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.