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Documenti fondamentali

GE17-0506-01

Sigma-Aldrich

Mono Q® HR 16/10

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About This Item

Codice UNSPSC:
41115700
NACRES:
SB.52

ligand

-OCH2CH(OH)CH2OCH2CH(OH)CH2N+(CH3)3

Materiali

PEEK column

Descrizione

Ion Exchanger Type (value)

Confezionamento

pkg of 1 ea

Parametri

435 psi

Dimensioni del letto

16 mm × 100 mm

Volume del letto

20 mL

Diam. int. colonna

16 mm

Matrice

monodisperse porous polystyrene/divinyl benzene beads
polymeric

Gruppo funzionale matrice

ammonium ion, quaternary phase

Dimens. media delle particelle

10 μm

cleaning

1-14

Intervallo di esercizio

2-12

Tecnica di separazione

anion exchange

Descrizione generale

Mono Q® HR 16/10 is a strong anion exchanger prepacked with MonoBeads. The column is an excellent choice for the polishing step in purification of proteins, peptides, and other biomolecules when high purity is required. The 20 mL bed volume of the column offers high capacity.

Caratteristiche e vantaggi

  • Monodisperse 10 μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
  • Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q ion exchangers.
  • Compatible with AKTA design, FPLC System, and other high-performance LC systems.
  • MonoBeads are also available as Mono P® for chromatofocusing

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Altre note

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Prodotti consigliati

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Note legali

FPLC is a trademark of Cytiva
Mono P is a registered trademark of Cytiva
Mono Q is a registered trademark of Cytiva

Pittogrammi

Flame

Avvertenze

Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


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Certificati d'analisi (COA)

Lot/Batch Number

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Articoli

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocolli

This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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