G8021

Monoclonal Anti-β-Galactosidase antibody produced in mouse

clone GAL-13, ascites fluid

• Sinonimo/i: Monoclonal Anti-β-Galactosidase
• Codice UNSPSC: 12352203
• NACRES: NA.46

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: ascites fluid
• Tipo di anticorpo: primary antibodies
• Clone: GAL-13, monoclonal
• contiene: 15 mM sodium azide
• Reattività contro le specie: bacteria
• tecniche: dot blot: suitable using soluble β-galactosidase; immunocytochemistry: suitable; indirect ELISA: 1:2,000 using mouse primary antibody, bridging antibody and Escherichia coli β-galactosidase
• Isotipo: IgG1
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: Escherichia coli ... lacZ(1036228) , lacZ(1036228) ; Escherichia coli K12 ... Lacz(945006)

Descrizione

Descrizione generale

Monoclonal Anti-β-Galactosidase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. β −galactosidase is a E coli protein and is a tetramer with 1023 amino acids.

Specificità

The antibody may be used for amplification in immunoenzymatic staining by preparing a β-galactosidase anti-β-galactosidase (BGABG) soluble complex. It is not recommended for immunoblotting; it does not recognize denatured or reduced β-galactosidase.

Immunogeno

β-galactosidase from E. coli

Applicazioni

Monoclonal Anti-β-Galactosidase antibody produced in mouse has been used in:

• immunostaining and histochemistry
• immunostaining and microscopy[1]
• immunohistochemistry
• immunofluorescence staining
• enzyme linked immunosorbent assay(ELISA)
• immuno dot blot

Azioni biochim/fisiol

β −galactosidase substrate is lactose. Synthetic substrates like o-nitrophenyl-β-d-galactopyranoside (oNPG) or p-nitrophenyl-β-d-galactopyranoside (pNPG) are used in enzymatic assays. It is also used as marker in studying gene expression. The use of monoclonal β -galactosidase anti- β -galactosidase (BGABG) soluble complex in the unlabeled antibody-enzyme method results in an intense signal with a very low background.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable