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31149

Sigma-Aldrich

Deoxyribonucleic acid, low molecular weight from salmon sperm

Sinonimo/i:

Salmon sperm dsDNA, low molecular weight DNA, DNA

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About This Item

Numero CAS:
100403-24-5
Numero CE:
309-566-6
Numero MDL:
MFCD00130921
Codice UNSPSC:
41106310
eCl@ss:
32160414
NACRES:
NA.52

Grado

for molecular biology
puriss. p.a.

Forma fisica

lyophilized powder

Impurezze

≤5% protein

Solubilità

50 mM Tris pH 8.0: 10 mg/mL, clear, faintly brown-yellow

Rapporto di assorbanza

A260/280 nm ~1.4

Temperatura di conservazione

2-8°C

Applicazioni

Various concentrations of Deoxyribonucleic acid, low molecular weight, from salmon sperm has been used:
  • to study DNA binding to silicon-substituted hydroxyapatite nanocrystals.
  • in simple electrochemical DNA biosensors to detect and evaluate DNA damage caused by UV-C radiation and reactive oxygen species.
  • as a precursor in the synthesis of the bio-dots.

Azioni biochim/fisiol

Salmon sperm DNA is often used as a blocking agent invarious research applications. It isknown to improve the specificity and sensitivity of the multi-AP assay byreducing non-specific binding and background signal.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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David Czeiger et al.
American journal of clinical pathology, 135(2), 264-270 (2011-01-14)
Elevated circulating cell-free DNA (CFD) levels were found in patients with cancer. The standard CFD assays are work-intensive and expensive. The aim was to evaluate in patients with cancer a new simple CFD assay. In mice inoculated with cancer cells
Jing Yu et al.
Molecular biology of the cell, 13(2), 558-569 (2002-02-21)
Dramatic changes occur in nuclear organization and function during the critical developmental transition from meiosis to mitosis. The Drosophila nuclear lamina protein YA binds to chromatin and is uniquely required for this transition. In this study, we dissected YA's binding
Andrea Hájková et al.
Bioelectrochemistry (Amsterdam, Netherlands), 116, 1-9 (2017-03-18)
A simple electrochemical DNA biosensor based on a glassy carbon electrode (GCE) was prepared by adsorbing double-stranded DNA (dsDNA) onto the GCE surface and subsequently used for the detection of dsDNA damage induced by hydroxyl radicals. Investigation of the mutual
Eiji Sunami et al.
Clinical chemistry, 55(3), 559-567 (2009-01-10)
Prostate cancer (PCa) detection using serum-based prostate specific antigen (PSA) is limited by frequent false-positive and -negative results. Genetic aberrations such as allelic imbalance (AI) and epigenetic changes such as promoter hypermethylation have been detected in circulating DNA of cancer
J J Gao et al.
Journal of immunology (Baltimore, Md. : 1950), 166(11), 6855-6860 (2001-05-22)
LPS is well recognized for its potent capacity to activate mouse macrophages to produce TNF-alpha, an important inflammatory mediator in bacterial infection-related diseases such as septic shock. We demonstrate here that while inducing only low levels of TNF-alpha alone, DNA
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