This kit provides sufficient reagents to perform 18 chloroplast isolation procedures, using 30 grams of starting plant tissue, as described in the protocol. Please see the link below to review the product datasheet:_x000D_
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/114/229/cpisopis-ms.pdf
CPISO
Chloroplast Isolation Kit
isolation of intact chloroplasts from leaves
Sinonimo/i:
Chloroplast Purification Kit, Isolation Kit for Chloroplast, Kit for Chloroplast Isolation
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1 KIT
CHF 831.00
CHF 831.00
Spedizione prevista il02 giugno 2025
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Cambia visualizzazione
1 KIT
CHF 831.00
About This Item
Codice UNSPSC:
12352200
NACRES:
NA.32
CHF 831.00
Spedizione prevista il02 giugno 2025
Prodotti consigliati
Livello qualitativo
tecniche
centrifugation: suitable
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
Descrizione generale
The Chloroplast Isolation Kit provides an efficient procedure for isolating intact chloroplasts from plant leaves. The procedure involves mechanical cell wall and cell membrane breakage, removal of cell debris and leaf tissue by filtration, collection of total cell chloroplast fraction by centrifugation, and separation of intact chloroplasts from broken chloroplasts using a Percoll® layer or gradient.
Applicazioni
Chloroplast Isolation Kit may be used in chloroplast organelle research to isolate intact chloroplasts from plant leaves for structural and functional studies.
Caratteristiche e vantaggi
- Specifically formulated buffer - Saves time using easy to follow simple and proven protocols
- Isolates intact chloroplasts - Useful for studies of such processes as carbon assimilation, electron flow and phosphorylation, metabolic transport or protein targeting
- Total organelle protein complement - Useful for functional studies, metabolic transport, proteomic profiling, and genomic applications
- Convenient format - Saves time and reduces waste
Compatibilità
The Chloroplast Isolation Kit has been tested for use with spinach, pea, lettuce, cabbage, mangold, and tobacco.
I componenti del kit sono disponibili anche separatamente
N° Catalogo
Descrizione
SDS
Codice della classe di stoccaggio
10 - Combustible liquids
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