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CHP2NC

Sigma-Aldrich

Imprint® Ultra Chromatin Immunoprecipitation Kit, Without Controls

ChIP kit for maximum sensitivity, compatible with Next-Gen sequencing

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About This Item

Codice UNSPSC:
41116158
NACRES:
NA.54

Descrizione generale

The Imprint Ultra Chromatin IP Kit is Sigma′s second generation chromatin immunoprecipitation (ChIP) kit developed for maximum sensitivity and optimum next-generation sequencing results. It provides a complete solution for Chromatin Immunoprecipitation, including columns and reagents for DNA purification. The kit allows researchers to explore the genome-wide binding sites of low abundance transcription factors (TFs), as well as novel histone modifications. It is optimized for ChIP reactions with chromatin from 106 cells (up to ~50 μg DNA), and can also be scaled up (or several preparations pooled) to accommodate 108 cells for genome-wide binding studies in ChIP-chip and ChIP-Seq applications.

Applicazioni

  • Suitable for downstream applications
  • Individual target characterization to genome-wide profiling techniques
  • Characterization of signal transduction pathways
  • Verification of ChIp-chIP and ChIP-seq data

Caratteristiche e vantaggi

  • Use successfully CHiP′ed DNA associated with low abundance, medium, and highly expressed transcription factors, as well as histone modifications
  • Greater capacity - can be used over a wide range of cell numbers ranging from 2-10 × 106
  • Maximum sensitivity - capable of detecting low abundance transcription factors with as little as 2 × 106 cells
  • Employs DNA-Blocked "Staph-Seq" for IP (immunoprecipitation), minimizing contaminating Staph A DNA in downstream ChIP-Seq applications.

Note legali

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Solo come componenti del kit

N° Catalogo
Descrizione
  • Sodium dodecyl sulfate solution, for molecular biology, 10% in 18 megohm water
  • Monoclonal Anti-POLR2A antibody produced in mouse, clone 1F17, purified immunoglobulin, buffered aqueous solution

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • S6576ChIP Next Gen Seq Sepharose™SDS
  • I8896IGEPAL® CA-630, for molecular biologySDS
  • S5150Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell cultureSDS
  • W4502Water, Nuclease-Free Water, for Molecular BiologySDS
  • I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powderSDS
  • I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powderSDS
  • A7638Bovine Serum Albumin, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)SDS
  • A84564-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)SDS
  • D9156Deoxyribonucleic acid, single stranded from salmon testes, For hybridizationSDS
  • M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solutionSDS
  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionSDS
  • R4642Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)SDS
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Avvertenze

Danger

Classi di pericolo

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

Organi bersaglio

Central nervous system

Codice della classe di stoccaggio

5.1A - Strongly oxidizing hazardous materials

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Comparison of sample preparation methods for ChIP-chip assays.
O'Geen, H., et al.
Biotechniques, 41, 557-557 (2006)
Chromatin isolation by RNA purification (ChIRP).
Chu, C., et al.
Journal of Visualized Experiments (2012)
Philippe Collas
Molecular biotechnology, 45(1), 87-100 (2010-01-16)
The biological significance of interactions of nuclear proteins with DNA in the context of gene expression, cell differentiation, or disease has immensely been enhanced by the advent of chromatin immunoprecipitation (ChIP). ChIP is a technique whereby a protein of interest
Genomic maps of long noncoding RNA occupancy reveal principles of RNA-chromatin interactions.
Chu, C., et al.
Molecular Cell (2011)
Genetic Framework for GATA Factor Function in Vascular Biology
Linnemann A.K., et al.
Proceedings of the National Academy of Sciences of the USA, 1, 13641-13646 (2011)
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Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

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