CHP2

Imprint^® Ultra Chromatin Immunoprecipitation Kit

Complete ChIP kit for sensitivity, compatibility with Next-Gen sequencing

• UNSPSC Code: 12352202

Descrizione

General description

The Imprint Ultra Chromatin IP Kit is Sigma′s second generation chromatin immunoprecipitation (ChIP) kit developed for maximum sensitivity and optimum next-generation sequencing results. It provides a complete solution for Chromatin Immunoprecipitation, including columns and reagents for DNA purification. The kit allows researchers to explore the genome-wide binding sites of low abundance transcription factors (TFs), as well as novel histone modifications. It is optimized for ChIP reactions with chromatin from 10⁶ cells (up to ~50 μg DNA), and can also be scaled up (or several preparations pooled) to accommodate 10⁸ cells for genome-wide binding studies in ChIP-chip and ChIP-Seq applications.

Application

• Suitable for downstream applications
• Individual target characterization to genome-wide profiling techniques
• Characterization of signal transduction pathways
• Verification of ChIp-chIP and ChIP-seq data

Features and Benefits

• Use successfully CHiP′ed DNA associated with low abundance, medium, and highly expressed transcription factors, as well as histone modifications
• Greater capacity - can be used over a wide range of cell numbers ranging from 2-10 × 10⁶
• Maximum sensitivity - capable of detecting low abundance transcription factors with as little as 2 × 10⁶ cells
• Employs DNA-Blocked "Staph-Seq" for IP (immunoprecipitation), minimizing contaminating Staph A DNA in downstream ChIP-Seq applications.

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Solo come componenti del kit

• Sodium dodecyl sulfate solution, 10% in 18 megohm water
• Monoclonal Anti-POLR2A antibody produced in mouse, clone 1F17, purified immunoglobulin, buffered aqueous solution

I componenti del kit sono disponibili anche separatamente

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• I5381

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• A7638

  Bovine Serum Albumin, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)

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Informazioni sulla sicurezza

• pictograms: GHS02,GHS03,GHS08,GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H226,H271,H290,H302,H314,H334,H336,H410
• pcodes: P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1A - STOT SE 3
• target_organs: Central nervous system
• Classe di stoccaggio: 5.1A - Strongly oxidizing hazardous materials