293-PERV-PK-CIRCE

97051411, human kidney, Not specified

• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: 293-PERV-PK-CIRCE, 97051411
• Origine biologica: human kidney
• Modalità di accrescimento: Adherent aggregates
• Cariotipo: Not specified
• Morfologia: Not specified
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human embryo kidney, infected with endogenous retrovirus of pigs

Descrizione della linea cellulare

293-PERV-PK-CIRCE has been established by infecting the human kidney 293 cell line (Sigma Catalogue number. 85120602) with retrovirus produced by the pig kidney cell line PK-15. The endogenous, C-type retrovirus particles produced by PK-15 have been shown to be capable of infection, replication and serial transfer. 293-PERV-PK-CIRCE is useful to examine the host range of the pig retrovirus to assess the risks associated with porcine xenotransplantation. The cell line has tested negative for Hog Cholera virus. Handle under ACDP Category 2 conditions.

Applicazioni

Pig retrovirus studies, infectivity assays

Terreno di coltura

DMEM + 2mM Glutamine + 10% HI-FBS

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:6 cells can be dislodged by mechanical agitation 5% CO₂ 3

Altre note

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