HT29 Cell Line human

91072201, human colon, Epithelial

• Sinonimo/i: HT29 Cells
• Codice UNSPSC: 41106514

Proprietà

• product name: HT29 Cell Line human, 91072201, Caucasian colon adenocarcinoma grade II
• Origine biologica: human colon
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 46, hypertriploid
• Morfologia: Epithelial
• Prodotti: Secretory component of Immunoglobulin A (IgA), Carcinoembryonic antigen (CEA)
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian colon adenocarcinoma

Descrizione della linea cellulare

Isolated from a primary tumour in a 44 year old Caucasian female. Forms a well-differentiated adenocarcinoma consistent with colony primary, grade I. Tumours also form in steroid treated hamsters. Has the following HLA profile A1,3; B12,17; Cw5.

Applicazioni

HT29 Cell Line has been used to determine viral titres of human parechovirus. It has also been used to study the ability of Bifidobacterium and Lactobacillus strains to counteract the toxic effect of C. difficile LMG21717.

Tumourigenicity studies

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 8,9
vWA: 17,19

Terreno di coltura

McCoy′s 5A + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% Trypsin; 5% CO₂; 37°C.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.