CTLL-2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93042610, mouse blood, Lymphoblast

• Sinonimo/i: CTLL-2 cell line
• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: mouse blood
• Confezionamento: tube of 5 μg 93042610-DNA-5UG; pkg of vial of cells 93042610-1VL
• Modalità di accrescimento: Suspension
• Cariotipo: Not specified
• Morfologia: Lymphoblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse C57b1/6 T cell

Descrizione della linea cellulare

CTLL is a subclone of T cells derived from a C57bl/6 mouse. The cells require IL-2 for growth and are used to assay for its presence in conditioned media.

Applicazioni

CTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines.[1][2]

Terreno di coltura

RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human Recombinant IL-2 + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Maintain cultures between 3-9 x 10⁵ cells/ml; 5% CO₂; 37°C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 4-5 x 10⁵ cells/ml.

Altre note

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