OLGA human cell line

IHW Number: 9100, 88052071

• Sinonimo/i: OLL
• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human blood
• Etnia: Native South American, Warao
• Genere: female
• Modalità di accrescimento: Suspension
• Cariotipo: Not specified
• Morfologia: Lymphoblastoid
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice

Descrizione

Descrizione generale

Consanguineous: Yes

Homozygous: Yes

Descrizione della linea cellulare

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Profilo DNA

A: 3101
Cw: 0102 0304
B: 1501 1520
HLA DRA: 102
HLA DRB1: 8022
HLA DQAP: 4.2
HLA DQA1: 401
HLA DQB1: 402
HLA DPA1: 1
HLA DPB1: 0301 0402

Profilo sierologico

HLA A: 31
HLA A ief: 31
HLA C: 1 10
HLA B: 62
HLA B ief: 62.3
Bw4/bw6: 6
HLA Dw: 8.2
HLA DR: 8
HLA DQ: 4
HLA DP: 3 4

Terreno di coltura

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Mantenimento delle subcolture

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Altre note

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Esclusione di responsabilità

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.