ZL55 was derived from an epithelial tumor of the pleural cavity of a 52-year old male, prior to treatment, with a known history of exposure to asbestos.
Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Suggested seeding density 1-4 x 10,000 cells/cm².
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