C9336

Anti-Chloramphenicol Acetyl Transferase (CAT) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Sinonimo/i: CAT Antibody - Anti-Chloramphenicol Acetyl Transferase (CAT) antibody produced in rabbit, Cat Antibody
• Numero MDL: MFCD01092314
• Codice UNSPSC: 12352203
• NACRES: NA.46

Proprietà

• Origine biologica: rabbit
• Coniugato: unconjugated
• Forma dell’anticorpo: IgG fraction of antiserum
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: buffered aqueous solution
• PM: antigen 26 kDa
• Convalida avanzata: recombinant expression; Learn more about Antibody Enhanced Validation
• tecniche: indirect immunofluorescence: 10 μg/mL using eukaryotic cells transfected with a plasmid bearing the CAT gene; western blot: 10 μg/mL using eukaryotic cells transfected with a plasmid bearing the CAT gene
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

Chloramphenicol Acetyl Transferases (CATs) shows conservation and differences in their amino acid sequences. CAT is encode by cat gene and exists as monomer and later assemble into a trimer.

Specificità

Anti-Chloramphenicol Acetyl Transferase (CAT) antibody is specific for bacterial CAT and recombinant CAT expressed in transfected eukaryotic cells (a predominant band of approx. 26 kD).

Immunogeno

bacterial chloramphenicol acetyl transferase (CAT).

Applicazioni

Anti-Chloramphenicol Acetyl Transferase (CAT) has been used in

• immunoblotting
• indirect immunofluorescence
• immunofluorescence microscopy

Azioni biochim/fisiol

Bacterial chloramphenicol acetyl transferase (CAT) is an enzyme that catalyzes the inactivation of the antibiotic, chloramphenicol, by acetylation and subsequently confers bacterial resistance to the antibiotic. CAT, being a stable prokaryotic enzyme, is often used as a reporter gene in transfection assays developed for eukaryotic promoters. Quantification of reporter gene expressions, such as that of CAT, can be correlated to the transcriptional functions of the target sequence. Thus, antibodies directed against CAT can be used for the study of gene sequences that are fused to the CAT reporter gene
Anti-Chloramphenicol Acetyl Transferase (CAT) antibody is specific for bacterial CAT and recombinant CAT expressed in transfected eukaryotic cells (a predominant band of approx. 26 kD). Staining of CAT by the antibody is inhibited by the bacterial CAT antigen in cells transfected with CAT.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable