It is recommended to reconstitute Chondroitinase ABC in a 0.1% BSA aqueous solution. Solubilizing 2 units in a 2 mL volume will result in a concentration of 2 units/mL or 0.002 units/µL.
C2905
Condroitinasi ABC
lyophilized powder, 0.3-3 units/mg solid
Sinonimo/i:
Condroitina ABC liasi
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2 UNITS
CHF 215.00
5 UNITS
CHF 429.00
10 UNITS
CHF 812.00
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Cambia visualizzazione
2 UNITS
CHF 215.00
5 UNITS
CHF 429.00
10 UNITS
CHF 812.00
About This Item
Prodotti consigliati
Origine biologica
bacterial (Proteus vulgaris)
Livello qualitativo
Coniugato
(Glucosaminoglycan)
Stato
lyophilized powder
Attività specifica
0.3-3 units/mg solid
PM
~120 kDa by gel filtration
Composizione
Protein, ~35% biuret
applicazioni
diagnostic assay manufacturing
Temperatura di conservazione
−20°C
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Categorie correlate
Applicazioni
Chondroitinase ABC from Sigma has been used to digest O-linked chondroitin sulfate-like glycosaminoglycan (CSGAG).[1] The enzyme has also been used to digest glycosaminoglycan chains in proteoglycans during the construction and expression of a recombinant versican gene in mammalian systems.[2] Furthermore, it has been used to digest chondroitin sulfate in the proteoglycan complexes.[3] The enzyme has also been used in the removal of glycan chains from aggrecan, which eliminates the axon growth-inhibitory effect of aggrecan.[4]
Azioni biochim/fisiol
Chondroitinase ABC catalyzes the eliminative degradation of polysaccharides containing (1-4)-β-D-hexosaminyl and (1-3)-β-D-glucuronosyl (or (1-3)-α-L-iduronosyl) linkages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groups. It acts on chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate. However, it acts slowly on hyaluronate. The molecular weight is approximately 120 kDa with 2 non-identical subunits of molecular masses 86 kDa and 32 kDa. The pH optimum is 8.0 with chondroitin sulfate and 6.8 with hyaluronic acid. The optimal temperature is 37 °C. 1 mM Zn2+ acts as an inhibitor, while 0.05 M acetate is an activator of the enzyme.
Confezionamento
Packages based on chondroitinase C activity.
Definizione di unità
One unit will liberate 1.0 μmole of a mixture of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-4-O-sulfo-D-galactose and 1.0 μmole of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-6-O-sulfo-D-galactose from chondroitin sulfate from shark cartilage, per min at pH 8.0 at 37 °C.
Stato fisico
Lyophilized powder containing Tris buffer salts
Nota sulla preparazione
Reconstitute in a 0.01% bovine serum albumin aqueous (BSA) solution. Subsequent dilutions can be made into a buffer containing 50 mM Tris, pH 8.0, with 60 mM sodium acetate and 0.02% BSA. Solutions should be freshly prepared.
Altre note
Per ulteriori informazioni sugli enzimi per l′analisi dei carboidrati complessi, visitare il sito web www.sigma-aldrich.com/enzymeexplorer.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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Articoli
Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
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Chondroitinase ABC from Proteus vulgaris C2905 2UNIT 용해 방법 문의 2unit Chondroitinase ABC을 0.002 unit/ul Chondroitinase ABC로 제조한다고 하면, 바로 완충용액 1ml를 넣어서 2unit/ml ->0.002unit/ul로 제조하는 것이 맞는지 확인요청드립니다.
1 answer-
Helpful?
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The specific activity range is 0.3-3unit/mg protein. I want to know something about it and how to use Product No. C2905.
1 answer-
The product datasheet contains additional information regarding this product, including usage instructions. Please see the link below to review this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/328/700/c2905dat-ms.pdf
To discuss further, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquires" under the Products Section with all the required information, including the specific questions, so that a member of our team can reach out to you to assist further. Thank you.
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How should Product No. C2905 be reconstituted?
1 answer-
Reconstitute the product in a 0.01% bovine serum albumin aqueous solution. Subsequent dilutions can be made into a buffer containing 50 mM Tris, pH 8.0, with 60 mM sodium acetate and 0.02% bovine serum albumin. Solutions should be prepared fresh.
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What is the composition of Product No. C2905?
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This product consists of approximately 35% protein as determined by the Biuret method. BSA (bovine serum albumin) is a major component (~90%) of the protein. The remaining 65% is Tris buffer salts.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight of this enzyme (Product No. C2905)?
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Chondroitinase ABC is a lyase with a reported molecular weight of approximately 120 kDa by gel filtration and is composed of two subunits with molecular weights of 86 and 32.
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