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H8891

Sigma-Aldrich

Heparinase III from Flavobacterium heparinum

Lyophilized powder stabilized with approx. 25% (w/w) bovine serum albumin, ≥30 units/mg protein (enzyme + BSA)

Sinonimo/i:

Heparin Lyase III, Heparitinase I, Heparitinase from Flavobacterium heparinum

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5 UNITS
CHF 253.00
10 UNITS
CHF 436.00
50 UNITS
CHF 1’730.00

CHF 253.00


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Cambia visualizzazione
5 UNITS
CHF 253.00
10 UNITS
CHF 436.00
50 UNITS
CHF 1’730.00

About This Item

Numero CAS:
Classificazione EC (Enzyme Commission):
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54

CHF 253.00


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Origine biologica

bacterial (Flavobacterium heparinum)

Livello qualitativo

Coniugato

conjugate (Glucosaminoglycan)

Stato

lyophilized powder

Attività specifica

≥30 units/mg protein (enzyme + BSA)

PM

70.8 kDa

applicazioni

diagnostic assay manufacturing

Temperatura di conservazione

−20°C

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Applicazioni

Heparinase III from Flavobacterium heparinum has been used to cleave specific GAGs.[1][2] It has been used to study its effect on the electrophoretic mobility of the protein reacting with the anti-aFGF (acidic fibroblast growth factor) antibody.[3]

Azioni biochim/fisiol

Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate.
Heparinase III prevents the formation of new blood vessels in vivo.[4] It specifically degrades heparin sulphate at the HNY-I and HNY,6X-G2 links. Unlike other heparinise, heparinase III does not contain the amino acid cysteine. It contains 13 histidine residues, which is responsible for its catalytic action.[5] This enzyme is not active towards heparin or low molecular weight heparins.

Definizione di unità

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units. Package sizes are sold in Sigma units.

Pittogrammi

Health hazard

Avvertenze

Danger

Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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Composition of the endothelial glycocalyx and its relation to its thickness and diffusion of small solutes
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A Constantinescu et al.
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A thick endothelial glycocalyx contributes to the barrier function of vascular endothelium in macro- and microcirculation. We hypothesised in the current study that diet-induced hyperlipidaemia perturbs the glycocalyx, resulting in decreased dimensions of this layer and increased transendothelial lipoprotein leakage
Andrea Garolla et al.
Human reproduction (Oxford, England), 27(4), 967-973 (2012-02-09)
Human papillomavirus (HPV) infection has been demonstrated in the sperm of a large percentage of sexually active males and is associated with an impairment of sperm parameters, with a particular negative impact on sperm motility, suggesting a possible role in
FGF binding by extracellular matrix components of Wharton's jelly
Malkowski A, et al.
Acta Biochimica Polonica, 54(2), 357-357 (2007)
Erin M Smith et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(51), 21683-21688 (2009-12-08)
Platelet-derived growth factor (PDGF) signaling is essential for processes involving cell motility and differentiation during embryonic development in a wide variety of organisms including the mouse, frog, zebrafish, and sea urchin. In early Xenopus laevis embryos, PDGF-AA provides guidance cues

Articoli

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Questions

1–3 of 3 Questions  
  1. Does Product No. H8891, Heparinase III, contain calcium?

    1 answer
    1. This product is lyophilized from a solution which is 75 mM NaCl/ 5mM NaH2PO4. We have no reason to believe that calcium ions are present.

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  2. Is Product No. H8891, Heparinase III sold based on Sigma Units or International Units?

    1 answer
    1. This material is sold based on Sigma Units.  One Sigma Unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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