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Key Documents

C-14056

Sigma-Aldrich

Human Tracheal Smooth Muscle Cells (HTSMC)

1 million cells per pellet

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About This Item

Codice UNSPSC:
41106514
NACRES:
NA.81

Origine biologica

human trachea

Confezionamento

pkg of 1,000,000 cells

Morfologia

(smooth muscle)

applicazioni

cell analysis (DNA, RNA, and protein)

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Descrizione generale

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Cells are dissolved in RNAlater for subsequent RNA, DNA, or protein analysis. Note that cell pellets cannot be revived.

Origine della linea cellulare

Trachea

Applicazioni

Primary Human Tracheal Smooth Muscle Cells (HTSMC) are isolated from the trachea from single donors and stain positive for smooth muscle α-actin. They connect the endings of the tracheal cartilage by a contractile bridge called the paries membranaceus and consequently regulate the airway lumen. Under pathological conditions like asthma, the amount of smooth muscle cells in the trachea walls is increased leading to smooth muscle hypertrophy or hyperplasia. Hence, HTSMC are suitable for in vitro studies of asthma and other pulmonary diseases.

Qualità

Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Attenzione

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Stato fisico

Pellet is dissolved in RNAlater® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.

Mantenimento delle subcolture

Click here for more information.

Altre note

The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.

Note legali

RNAlater is a registered trademark of Ambion, Inc.

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

Smooth muscle human primary cells isolated from blood vessels and hollow organs, plus smooth muscle cell growth media. Protocols on media handling, cell passaging and product use.

Primary cells from PromoCell are available in pelleted form for research applications like gene expression analysis, and cell/protein characterization experiments.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.