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Merck
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Documenti fondamentali

A9275

Sigma-Aldrich

Avidin from egg white

BioUltra, lyophilized powder, ≥10 units/mg protein (E1%/280), ≥98% (SDS-PAGE)

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About This Item

Numero CAS:
Numero CE:
Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.46
Prezzi e disponibilità al momento non sono disponibili

Origine biologica

egg white

Nome Commerciale

BioUltra

Saggio

≥98% (SDS-PAGE)

Stato

lyophilized powder

Attività specifica

≥10 units/mg protein (E1%/280)

PM

glycoprotein 66 kDa
subunit 16 kDa

Temperatura di conservazione

2-8°C

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Descrizione generale

Avidin protein is a homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues[1]. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Applicazioni

Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.
Egg white avidin has been used as a cross-linker for biotin-conjugated antibodies[2]. The product has also been used as a blocking agent in ELISA applications[3].

Definizione di unità

One unit will bind 1.0 μg of d-biotin.

Nota sulla preparazione

Affinity purified

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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