Biotin is soluble directly in water at 0.22 mg/mL, a 1 mg/mL solution is water is not achievable. However, this product is tested for solubility at 50 mg/mL in 2 N NaOH. Concentrated stock solutions prepared in NaOH may then be diluted in buffer, medium, or water to the desired concentration. The solution stability, when stored at ?20 °C, has not been investigated. The end user would have to determine stability. As a general practice, avoid repeated freeze/thaw cycles. Note that some versions of classical medium, such as Ham’s F12 (see product N6658) include D-Biotin. These sterile liquid media are stable for approximately 1 year at 2–8 °C. Similar stability would be expected for sterile Biotin stock solutions.
B4639
Biotina
powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Sinonimo/i:
Biotina-D, Bios II, Coenzima R, Vitamina B7, Vitamina H
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Cambia visualizzazione
| Voi/SKU | Disponibilità | Prezzo |
|---|---|---|
100 mg | Disponibile per la spedizione OGGIdaSchnelldorf | CHF 49.90 |
500 mg | Disponibile per la spedizione OGGIdaSchnelldorf | CHF 211.00 |
1 g | Disponibile per la spedizione OGGIdaSchnelldorf | CHF 387.00 |
5 g | Disponibile per la spedizione OGGIdaSchnelldorf | CHF 1210.00 |
Informazioni su questo articolo
Formula empirica (notazione di Hill):
C10H16N2O3S
Numero CAS:
Peso molecolare:
244.31
NACRES:
NA.21
PubChem Substance ID:
eCl@ss:
34058011
UNSPSC Code:
12352201
EC Number:
200-399-3
MDL number:
Beilstein/REAXYS Number:
86838
Servizio Tecnico
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BioReagent
Quality Segment
assay
≥99%
form
powder
mol wt
Mw 244.31 g/mol
technique(s)
cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable
color
white to off-white
mp
231-233 °C (lit.)
solubility
H2O: 0.2 mg/mL (Solubility increases with addition of 1 N NaOH.)
storage temp.
2-8°C
SMILES string
[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2
InChI
1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1
InChI key
YBJHBAHKTGYVGT-ZKWXMUAHSA-N
General description
La biotina è anche chiamata complesso della vitamina B. È un composto biciclico che ha peso molecolare 244,31 kDa. La biotina agisce come un cofattore importante per le carbossilasi nei mammiferi. Si trova all′interno delle cellule in riserve metaboliche libere e legate.
Application
La biotina è stata usata:
- come supplemento M199 del terreno nel sistema di coltura in vitro
- come componente di un terreno SATO privo di siero per l′isolamento di precursori corticali oligodendrocitici (OPCs)
- in colture di precursori biochimici per sintesi di acidi grassi de novo
- come componente in terreni di coltura aggregati
Biochem/physiol Actions
Vitamina essenziale che è importante per il metabolismo di aminoacidi ed energia e la sintesi di acidi grassi. È un gruppo prostetico in quattro famiglie di carbossilasi nei mammiferi e facilita il legame ed il trasferimento di anidride carbonica.
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Questo articolo | |||
|---|---|---|---|
| description powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99% | description certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland | description tested according to Ph. Eur. | description European Pharmacopoeia (EP) Reference Standard |
| technique(s) cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable | technique(s) LC/MS: suitable, gas chromatography (GC): suitable, HPLC: suitable | technique(s) - | technique(s) liquid chromatography (LC): suitable, gas chromatography (GC): suitable |
| assay ≥99% | assay - | assay - | assay - |
| form powder | form - | form - | form solid |
| solubility H2O: 0.2 mg/mL (Solubility increases with addition of 1 N NaOH.) | solubility - | solubility - | solubility - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - | storage temp. - |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level - |
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Classe di stoccaggio
11 - Combustible Solids
Che succede?
WGK 1
Punto di infiammabilità (°F)
Not applicable
Punto di infiammabilità (°C)
Not applicable
DPI (dispositivi di protezione individuale)
Eyeshields, Gloves, type N95 (US)
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Can I dilute it in H2O for 1mg/ml stock concentration, aliquot and store at -20C?
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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If I would like to make 1M biotin stock (B4639), what would be the best way?
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A 1 M solution of biotin would require a concentration of 244 mg/mL. The maximum solubility tested for this compound is 50mg/ml in 2M NaOH or NH4OH, which results in a 204 mM solution. Similar rates have been reported for DMSO. Heat and agitation may improve the solubility. However, a precipitate may form upon cooling. A search of the literature did not reveal a more efficient solvent for Biotin. The preparation of a 1M solution would have to be determined experimentally.
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Hello, How can I reconstruct biotin powder (B4639)? which solvent? and how can I prepare 50mM biotin solution for 1 ml final solution?
1 answer-
This product is soluble in 50 mg/ml 2 M NH4OH.
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