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A5545

Sigma-Aldrich

Anti-Guinea Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Sinonimo/i:

Rabbit Anti-Guinea pig IgG (whole molecule)−HRP

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1 ML
CHF 224.00

CHF 224.00


Spedizione prevista il21 maggio 2025


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1 ML
CHF 224.00

About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.46

CHF 224.00


Spedizione prevista il21 maggio 2025


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Origine biologica

rabbit

Coniugato

peroxidase conjugate

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Stato

buffered aqueous solution

Reattività contro le specie

guinea pig

tecniche

direct ELISA: 1:50,000-1:80,000
dot blot: 1:50,000-1:100,000 (chemiluminescent)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400-1:800

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

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Descrizione generale

An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. It has two subclasses of IgG- IgG1 and IgG2.

Specificità

Rabbit anti-guinea pig IgG (whole molecule)-peroxidase antibody binds to guinea pig IgG.

Immunogeno

guinea pig IgG

Applicazioni

Anti-Guinea Pig IgG (whole molecule) Peroxidase antibody produced in rabbit has been used in diphtheria serology enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

Azioni biochim/fisiol

IgGs are glycoprotein antibodies that modulate several immune responses. Guinea pig IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-guinea pig IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Immunoglobulin G (IgG) is the major class and most abundant immunoglobulin of immune system released by plasma cells. IgG mediate immune response with respect to the type of antigen and is found in blood or extracellular fluid. IgG defends against bacterial and viral infections. Maternal IgG crosses from mother to fetus through the placental barrier by pinocytosis to provide passive immunity for the neonate and protect against infections.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. IgG1 helps in complement fixation through alternate pathway and IgG2 fixes complement by conventional mechanism. It mainly helps in immune defense. Guinea Pig is a highly preferred laboratory animal to study the local and anaphylactic reactions.

Stato fisico

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Nota sulla preparazione

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Stoccaggio e stabilità

Store at -20 °C for long term use. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots at -20 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Skin Sens. 1

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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