The specific method used to test this material for protease activity is considered proprietary. However, it is based on the enzymatic cleavage of FITC-Casein. See the link below to review the FITC-Casein Enzymatic Assay to demonstrate the principle of this method.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/353/850/c3777enz.pdf
A3059
Sieroalbumina
heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%
Sinonimo/i:
Albumina bovina, Albumina siero bovino, BSA
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Cambia visualizzazione
| Voi/SKU | Disponibilità | Prezzo |
|---|---|---|
50 g | Per conoscere la disponibilità, visualizza il carrello | CHF 431.00 |
100 g | Per conoscere la disponibilità, visualizza il carrello | CHF 772.00 |
500 g | Per conoscere la disponibilità, visualizza il carrello | CHF 3230.00 |
Informazioni su questo articolo
Numero CAS:
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.27
MDL number:
eCl@ss:
32160409
Biological source:
bovine
Assay:
≥98%
Form:
lyophilized powder
Technique(s):
ELISA: suitable, western blot: suitable
Impurities:
essentially globulin free, protease, none detected
Servizio Tecnico
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Permettici di aiutartibiological source
bovine
Quality Segment
assay
≥98%
form
lyophilized powder
purified by
heat shock fractionation
packaging
poly bottle of
origin
USA origin
technique(s)
ELISA: suitable, western blot: suitable
impurities
essentially globulin free, protease, none detected
loss
≤5%
pH
7
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
BT Virus, none detected, VSV Virus, none detected
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
General description
Bovine serum albumin (BSA) has an important role in the regulation of blood colloidal osmotic pressure. BSA is the major protein in bovine serum, and is composed of 583 amino acids with a calculated molecular weight of 66,430 Daltons. BSA is used in many different applications and fields, such as:
This particular BSA is specifically evaluated and tested to have very low protease content / for protease-free status. Protease-free BSA may be used in applications like:
b) Nucleic acid hybridization
c) Radioimmunoassay (RIA)
- Protein quantitation (as a standard)
- Immunochemistry (as a blocking agent)
- Cell culture (as a nutrient)
This particular BSA is specifically evaluated and tested to have very low protease content / for protease-free status. Protease-free BSA may be used in applications like:
- nei saggi ELISA
- nei saggi ELISA
- Protease-sensitive techniques that include:
b) Nucleic acid hybridization
c) Radioimmunoassay (RIA)
Application
Albumina da siero bovino viene utilizzata per misure in luminescenza.
Biochem/physiol Actions
Si sospetta che alcuni epitopi conformazionali e di sequenza primaria di BSA siano allergeni nelle allergie umane a carne bovina e latte.
Features and Benefits
- Ottenuta mediante frazionamento per shock termico
- Assenza di proteasi
- Essentially globulin-free
Preparation Note
Ci si riferisce alla sieroalbumina come Frazione V. Questa designazione è presa dal metodo originale introdotto da Cohn per frazionare siero proteine usando la precipitazione in etanolo a freddo. La sieroalbumina è stata trovata da Cohn nella quinta frazione col suo metodo basato sull′etanolo. Da allora il termine “Frazione V” è stato usato da alcuni a prescindere dal metodo di preparazione. Altri hanno usato questa terminologia riferita a sieroalbumina purificata con metodi di frazionamento basati su etanolo che sono stati profondamente modificati dai tempi in cui Cohn li ha descritti. Sigma-Aldrich produce e distribuisce sieroalbumine purificate con vari metodi primari inclusi il metodo originale di frazionamento di Cohn, metodi modificati di frazionamento con etanolo, shock termico e cromatografia. Stadi successivi di purificazione possono comprendere cristallizzazione o filtrazione su carbone attivo.
Preparata mediante frazionamento termico
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Questo articolo | |||
|---|---|---|---|
| description heat shock fraction, protease free, essentially globulin free, pH 7, ≥98% | description lyophilized powder, essentially fatty acid free and essentially globulin free, ≥99% (agarose gel electrophoresis) | description heat shock fraction, protease free, fatty acid free, essentially globulin free, pH 7, ≥98% | description lyophilized powder, essentially globulin free, low endotoxin, ≥98% (agarose gel electrophoresis) |
| assay ≥98% | assay ≥99% (agarose gel electrophoresis) | assay ≥98% | assay ≥98% (agarose gel electrophoresis) |
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| biological source bovine | biological source bovine | biological source bovine | biological source bovine |
| technique(s) ELISA: suitable, western blot: suitable | technique(s) enzyme immunoassay: suitable | technique(s) ligand binding assay: suitable, blood typing: suitable, ELISA: suitable | technique(s) ELISA: suitable |
| Quality Level 300 | Quality Level 300 | Quality Level 300 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
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Classe di stoccaggio
11 - Combustible Solids
Punto di infiammabilità (°F)
Not applicable
Punto di infiammabilità (°C)
Not applicable
DPI (dispositivi di protezione individuale)
Eyeshields, Gloves, type N95 (US)
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Which method do you use to determine the protease concentration?
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Helpful?
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What is the fatty acid content of Product A3059, Albumin from bovine serum (BSA)? Can it be considered fatty acid free?
1 answer-
The fatty acid content of A3059 is not tested in quality control, and it is not reported on our Certificate of Analysis. We would not refer to this product as "fatty acid free".
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Is Product A3059, Albumin from bovine serum (BSA), acetylated to inactivate nucleases?
1 answer-
A3059 is not acetylated. We do not test for the presence of nucleases. Acetylated BSA products would be B2518 or B8894.
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Is Product A3059, Albumin from bovine serum (BSA), suitable for use as a blocking agent in immunoblotting?
1 answer-
We have not tested A3059 for specific use in immunoblotting as a blocking agent, in quality control. However, A3059 is considered to be essentially gamma-globulin free, which makes this more potentially useful in blotting experiments, by reducing a potential source of background. The Technical Bulletin states that "Globulin free albumins are suitable for use in applications where no other proteins should be present".
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Product A3059, Albumin from bovine serum (BSA), suitable for use in ELISA?
1 answer-
We have not tested A3059 for specific use in ELISA. However, A3059 is considered to be essentially gamma-globulin free, which makes this more potentially useful in ELISA, by reducing a potential source of background.
Helpful?
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Is Product A3059, Albumin from bovine serum (BSA), endotoxin-free?
1 answer-
We have not tested A3059 for endotoxin content. Endotoxin-tested BSA products would include A4919, A9306, A9430, A2934, A9306, A9543, or A3675.
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