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Imidazole buffer Solution

BioUltra, 1 M in H2O

Sinonimo/i:

Imidazole solution, Glyoxaline solution

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Voi/SKUDisponibilitàPrezzo
100 mL
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CHF 130.00
500 mL
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CHF 511.00

Informazioni su questo articolo

Formula empirica (notazione di Hill):
C3H4N2
Numero CAS:
Peso molecolare:
68.08
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
103853
eCl@ss:
39161001

CHF 130.00


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product line

BioUltra

Quality Segment

form

liquid

concentration

1 M in H2O

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

pH

9.5±0.5

density

1.01 g/mL at 20 °C

SMILES string

c1c[nH]cn1

InChI

1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)

InChI key

RAXXELZNTBOGNW-UHFFFAOYSA-N

General description

Imidazole is used as a buffer in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for the assay of horseradish peroxidase and as a chelator for the binding of various divalent cations. Imidazole aids the elution of histidine-containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for the detection of proteins.

Application

Imidazole buffer solution has been used as a component:
  • of calmodulin-binding buffer 1 (CBB1) for the tandem affinity purification (TAP) of TAP-tagged proteins together with co-purifying proteins
  • of lysis buffer, and SDS loading buffer for His-ubiquitin pull-down assays
  • of imidazole wash and elution buffer for protein purification


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Questo articolo
792527RDD039I2399
Imidazolo anhydrous, free-flowing, Redi-Dri™, ACS reagent, ≥99%

Sigma-Aldrich

792527

Imidazolo

Imidazolo Molecular Biology, ≥99% (titration), free-flowing, Redi-Dri™

Sigma-Aldrich

RDD039

Imidazolo

Imidazolo ACS reagent, ≥99% (titration)

Sigma-Aldrich

I2399

Imidazolo

description

BioUltra, 1 M in H2O

description

anhydrous, free-flowing, Redi-Dri™, ACS reagent, ≥99%

description

Molecular Biology, ≥99% (titration), free-flowing, Redi-Dri™

description

ACS reagent, ≥99% (titration)

concentration

1 M in H2O

concentration

-

concentration

-

concentration

-

product line

BioUltra

product line

Redi-Dri™

product line

Redi-Dri™

product line

-

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

300

form

liquid

form

solid

form

crystalline powder

form

-

pH

9.5±0.5

pH

9.5-11.0 (25 °C, 5% in H2O)

pH

6.2-7.8 (25 °C)

pH

9.5-11.0 (25 °C, 5% in H2O)

density

1.01 g/mL at 20 °C

density

-

density

-

density

-


Pittogrammi

Health hazardCorrosion

Parola di avvertenza

Danger

Codici di pericolo

Hazard Classifications

Eye Dam. 1 - Repr. 1B - Skin Corr. 1C

Classe di stoccaggio

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Che succede?

WGK 2

Punto di infiammabilità (°F)

Not applicable

Punto di infiammabilità (°C)

Not applicable

DPI (dispositivi di protezione individuale)

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

Reviews

Active Filters

    • Review 1
    • Votes 0
    5 out of 5 stars.

    Brilliant

    This is brilliant product.
    Save me a lot of time to prepare endotoxin free buffer.

    Helpful?

  1. 2 Ratings-only reviews