RDD039

Imidazolo

for molecular biology, ≥99% (titration), free-flowing, Redi-Dri^™

• Sinonimo/i: 1,3-diaza-2,4-ciclopentadiene, Gliossalina
• Formula empirica (notazione di Hill): C₃H₄N₂
• Numero CAS: 288-32-4
• Peso molecolare: 68.08
• Beilstein: 103853
• Numero MDL: MFCD00005183
• Codice UNSPSC: 12352200
• NACRES: NA.21

Proprietà

• Grado: Molecular Biology; for molecular biology
• Livello qualitativo: 100
• Tensione di vapore: <1 mmHg ( 20 °C)
• Nome Commerciale: Redi-Dri^™
• Saggio: ≥99% (titration)
• Stato: crystalline powder
• Qualità: free-flowing
• pH: 6.2-7.8 (25 °C)
• pKa (25 °C): 6.95
• P. ebollizione: 256 °C (lit.)
• Punto di fusione: 88-91 °C (lit.)
• applicazioni: diagnostic assay manufacturing
• Attività estranea: DNase, RNase and protease, none detected
• Stringa SMILE: c1c[nH]cn1
• InChI: 1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5); RAXXELZNTBOGNW-UHFFFAOYSA-N

Descrizione

Descrizione generale

Imidazole is a heterocyclic compound with a five-membered planar ring. It is amphoteric and highly polar.[1]

Applicazioni

Ottimo per tamponi nell′intervallo di pH 6,2-7,8

Imidazole has been used

• in the lysis, wash, and elution buffer for the purification of histidine tagged proteins[2]
• in gradient for the purification of histidine tagged proteins using nickel affinity chromatography[3]
• as a component of homogenization buffer for the purification of phagosomal compartments from dendritic cells[4]

Azioni biochim/fisiol

Imidazole derivatives have antibacterial, antifungal and anticancer functionality.[1] It interacts with DNA and also binds to protein and stops cell division. It also acts as a microtubule destabilizing agents and inhibits topoisomerase and Cytochrome P450 Family 26 Subfamily A Member 1 (CYP26A1) enzymes. Imidazole based anticancer drug find applications in cancer chemotherapy.[5] It is used as buffer component for purification of the histidine tagged recombinant proteins in immobilized metal-affinity chromatography (IMAC).[6]

Caratteristiche e vantaggi

• Qualified for molecular biology applications, DNase, Rnase, and protease free
• Redi-Dri^™ packaging controls moisture, eliminating clumps
• Crystalline powder remains free-flowing and much easier to use
• Safer to handle and weigh out for routine use in lab
• Less waste, loss of drying, while maintaining high quality

Altre note

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Note legali

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Informazioni sulla sicurezza

• Pittogrammi: GHS08,GHS05,GHS07
• Avvertenze: Danger
• Indicazioni di pericolo: H302,H314,H360D
• Consigli di prudenza: P260 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classi di pericolo: Acute Tox. 4 Oral - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
• Codice della classe di stoccaggio: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): 293.0 °F - closed cup
• Punto d’infiammabilità (°C): 145 °C - closed cup