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38733

Laemmli Lysis-buffer

non smelling

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Informazioni su questo articolo

NACRES:
NA.25
UNSPSC Code:
12161700
Prezzi e disponibilità al momento non sono disponibili
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Quality Segment

form

liquid

composition

bromophenol blue, 0.004% , DTT, 400 mM , glycerol, 20% , SDS, 4% , TRIS, 0.125 M

pH

~6.8

suitability

in accordance for electrophoresis test

storage temp.

−20°C

Application

Laemmli Lysis-buffer is an non-smelling lysis buffer used to prepare protein samples for SDS gel electrophoresis.

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Questo articolo
B5020B4895P2192
10X PCR Buffer Optimized for routine PCR with MgCl2 included

Sigma-Aldrich

P2192

10X PCR Buffer

description

non smelling

description

pH 4.00±0.01 (25 °C)

description

pH 10.00±0.01 (25 °C)

description

Optimized for routine PCR with MgCl2 included

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

300

form

liquid

form

liquid

form

liquid

form

liquid

pH

~6.8

pH

4.00±0.01 (25 °C)

pH

10.00±0.01 (25 °C)

pH

-

storage temp.

−20°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

−20°C

suitability

in accordance for electrophoresis test

suitability

-

suitability

-

suitability

-

composition

SDS, 4% , bromophenol blue, 0.004% , glycerol, 20% , DTT, 400 mM

composition

-

composition

-

composition

-


pictograms

CorrosionExclamation mark

signalword

Danger

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B

Classe di stoccaggio

8B - Non-combustible corrosive hazardous materials



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Questions

1–7 of 7 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the advantage of using Laemmli Lysis-buffer, Product No. 38733?

    1 answer
    1. The odor of 38733 is not nearly as strong as that of S3401 (which has 2-mercaptoethanol).  Some people are very sensitive to the odor of 2-mercaptoethanol.

      Helpful?

  4. Is Laemmli Lysis-buffer, Product No. 38733, a 1× or a 2× solution?

    1 answer
    1. For most applications, it is considered to be a 2X solution.

      Helpful?

  5. What is the difference between the two Laemmli buffers S3401 and 38733?

    1 answer
    1. Product No. S3401 is 0.125 M Tris (pH 6.8) in 80% water/20% glycerol, with 4% SDS, 10% 2-mercaptoethanol and 0.004% bromophenol blue. 38733 is 0.125 M Tris in 80% water/20% glycerol, with 4% SDS, 400 mM DTT and 0.004% bromophenol blue.  So the only difference is the reducing agent; 2-mercaptoethanol (strong odor) vs. the DTT (not nearly as strong an odor).

      Helpful?

  6. Why do the Laemmli sample buffers like S3401 and 38733 have glycerol in them?

    1 answer
    1. An aqueous buffer with 20% glycerol is denser than one without. So when the sample is added to the well of an electrophoresis gel, it tends to stay in the well.

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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