56748

Imidazolo

BioUltra, Molecular Biology, ≥99.5% (GC)

• Sinonimo/i: 1,3-diaza-2,4-ciclopentadiene, Gliossalina
• Formula condensata: C₃H₄N₂
• Numero CAS: 288-32-4
• Peso molecolare: 68.08
• NACRES: NA.25
• PubChem Substance ID: 57651596
• eCl@ss: 39161001
• UNSPSC Code: 12352005
• EC Number: 206-019-2
• MDL number: MFCD00005183
• Beilstein/REAXYS Number: 103853

Proprietà

• grade: Molecular Biology
• Quality Segment: 200
• vapor pressure: <1 mmHg ( 20 °C)
• product line: BioUltra
• assay: ≥99.5% (GC)
• form: flakes, powder or crystals
• impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.2% water
• ign. residue (900 °C): ≤0.05%
• pH: 9.5-11.0 (25 °C, 0.1 M in H₂O)
• pKa (25 °C): 6.95
• bp: 256 °C (lit.)
• mp: 88-91 °C (lit.)
• solubility: H₂O: 0.1 M at 20 °C, clear, colorless
• anion traces: chloride (Cl^-): ≤50 mg/kg, sulfate (SO₄^2-): ≤50 mg/kg
• SMILES string: c1c[nH]cn1
• cation traces: Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg
• λ: 0.1 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.10, λ: 280 nm A_max: 0.10
• InChI: 1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)
• InChI key: RAXXELZNTBOGNW-UHFFFAOYSA-N

Descrizione

Application

Ottimo per tamponi nell′intervallo di pH 6,2-7,8

Imidazole can be used to prepare buffers in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for assay of horseradish peroxidase and as chelator for the binding of various divalent cations. Imidazole can be used for the elution of histidine containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for detection of proteins.

Other Notes

Review: As RNase model

Informazioni sulla sicurezza

• Pittogrammi: GHS08,GHS05,GHS07
• Parola di avvertenza: Danger
• Codici di pericolo: H302,H314,H360D
• Avvertenze di sicurezza: P260 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
• Classe di stoccaggio: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 2
• Punto di infiammabilità (°F): 293.0 °F - closed cup
• Punto di infiammabilità (°C): 145 °C - closed cup
• DPI (dispositivi di protezione individuale): Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges