318337

Acridine Orange hydrochloride hydrate

≥98% (HPLC)

• Sinonimo/i: 3,6-Bis(dimethylamino)acridine hydrochloride
• Formula empirica (notazione di Hill): C₁₇H₁₉N₃ · HCl · xH₂O
• Numero CAS: 65-61-2
• Peso molecolare: 301.81 (anhydrous basis)
• Colour Index: 46005
• Beilstein: 3734978
• Numero CE: 200-614-0
• Numero MDL: MFCD00150070
• Codice UNSPSC: 12171500
• ID PubChem: 24859123
• NACRES: NA.47

Proprietà

• Livello qualitativo: 200
• Saggio: ≥98% (HPLC)
• Forma fisica: powder
• Colore: orange
• Punto di fusione: 284-287 °C (lit.)
• Solubilità: H₂O: 0.1%, clear, orange to red
• λmax: 492 nm
• ε (coefficiente d’estinzione): ≥14,000 at 227-233 nm in water at 0.005 g/L; ≥35000 at 265-271 nm in water at 0.005 g/L
• applicazioni: diagnostic assay manufacturing; hematology; histology
• Temperatura di conservazione: room temp
• Stringa SMILE: Cl[H].[H]O[H].CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C
• InChI: 1S/C17H19N3.ClH.H2O/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;/h5-11H,1-4H3;1H;1H2; JUAAUEXLEKAEQC-UHFFFAOYSA-N

Descrizione

Applicazioni

A rigorously purified zinc-free material suitable for use in flow cytometry.

Acridine Orange hydrochloride hydrate dye has been used:

• to stain intracellular acidic vesicles.
• to measure lysosomal membrane permeability (LMP).
• or autophagy detection.

Fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Has been used to study apoptosis in various cell culture and tissue models.

Azioni biochim/fisiol

Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentrations, it intercalates into DNA and precipitates RNA. However, at high concentrations it denatures and precipitates both RNA and DNA. Acridine Orange is also used to analyze autophagy. It enters into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in acidic organelles giving a bright red fluorescence.

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Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Warning
• Indicazioni di pericolo: H341
• Consigli di prudenza: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classi di pericolo: Muta. 2
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)